Sequences from confirmation amplicon


Confirmation sequences for line 625F11 and hit At1g54080 are shown.
Line ID 625F11
Locus At1g54080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20186757
Primers used for PCR 22oh - 8474, show primer details
   
Sequence Name 14-K114815-0022-625-F11C-22oh
GenBank Accession LN492622 [GenBank]
Sequencing primer gene specific primer, 22oh: 5'-AAACAAAACGAACAAACATAAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114815-0022-625-F11C-22oh
AGTAGAGATATGTTTTTAGTGCAGTTTCGTCANCAAAGAAAATCAAAATTTCTAAGTATA
TATTAGTTTGGGCTGGGCCGTCAACAATTGGTCAGTCACTGAAACGAATTTGTTCGGGCC
TTGGGCTTGATCCGTCAACAACATCAAGGTTCAGAACAAATGATTGGTCAGTCACTAAAA
CGAATATCACAGCTGCCGTGAGATTAGTATACAGTCTATCTCTCTCTCGAAGAAATCGTC
ACACTCCTATAAATGGTCACTTGTTTCGTACGGCCTTTCTTTACTCCTTTAGAGAGAGCG
TGCCATTTTTATTTTTCTCTTTCTTCTCTCATTTTTATTTTCTTTTCTTTTTTTTCACCT
TTTTTTTTCCCTTTTTTTTCCTTTCTCCTCCTCCTAACTGGATTTTAAACCCTACCTAAA
GGGAAATTTTCTCGGGAAACAAAAAAAATTTTTTTATCCCATTGAGAAAAAACCCCAAAA
ATTGCAAAATCAAAGGCTTATTAAGCAGCAACAACAACAACAACAACGGCAACTTCAACA
AGCTTTAATTCAACAACCTATAATGGCTTCTTGTCCGGAAAATCTACTTGGATCACCATT
GAGTATAATGGTCAATATGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTTCATTTCAA
AAATGTAA
   
Sequence Name 06-K114814-0022-625-F11C-8409
GenBank Accession LN488924 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114814-0022-625-F11C-8409
ATCATAGCTTGTTGATGTTGCTGTTGTTGTTGTTGTTGTTGCTGCTTAATAAGCCTTTGA
TTCTGCATTTTTTGTGTTTCTTTCTCACTGCGATAAAAATATCTCTTTTGTTTCCCGAGA
AAATTCCCCTTAAGCTAGGGTTCAAAATCAAGTAAGAAGAAGAAGAAAGAAAAAAAAAGG
AAAAAAAAAGGGTGAAAAAAAAGAAAAGAAAATAAAATTGAGAGAAGAAAGAGAAAAATA
AAAATGGCACGCTCTCTCTAAAGGAGTAAAAAAAGGCCGTACAAAACAAGTGACCATTTA
TAGGAGTGTGACAATTTCTTCAAAAAAAAAATAAACTGTATACTAATCTCACGGCAGCTG
TGATATTCGTTTTAGTGACTGACCAATCATTTGTTCTGAACCTTGATGTTGTTGACGGAT
CAAGCCCAAGGCCCAAACAAATTCTTTTCATTGACTGACCAATTGTTGACGGCCCACCCC
AAACTAATATATACTTAAAAATTTTGATTTTCTTTGATTGACTGAAACTGCACTAAAAAA
CATTATCTTCTAACATAATTCATTGTTTGTTTTACCCCTAATGTTTGT

Last Updated on 06.12.2018 16:11