Sequences from confirmation amplicon


Confirmation sequences for line 625G03 and hit At3g09620 are shown.
Line ID 625G03
Locus At3g09620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2950510
Primers used for PCR NG70 - 8474, show primer details
   
Sequence Name 58-K102368-0022-625-G03C-8409
GenBank Accession LN509664 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K102368-0022-625-G03C-8409
GCCATTTACAATTGAATATATCCTGCCGCCGACAGAGGTCAAAACCCATATCAAACATAC
GATCAGCTTCATCCATTACCAAATATGTGACCCTTCGTAGATTGGTAATTTTTCCACTGC
TTGTGCAAAGAATATCGATCATTCTTCCAGGAGTACAAACAACAATCTCGGTACCTCGCT
TAAGCTCACTGATTTGCTGGGCAACTCCAGATCCTCCATACACAGGCACACATATTATAC
CCAATGCCTTAGAAAACTTTCTGATATCGCTGTAAATCTGCTGAACAAGTTCTCTAGTAG
GTGCCATTACAAGCCCAATCGGCCCATCACCAGCTTCAACGGGAGGCTGATCTTTGATAT
GCCTCAACATAGGCAAAACAAAGCCAAGGGTTTTACCTGACCCGGTTTTGGCAACCCCAA
TGCAGTCTCTACCGCTCATGATGATTGGAAGTGCTTGTGCTTGGATAGGCATTGGCTTTT
CATAGTTAAGCTTCTTCAGAGTATCCAAAATTTTGCTAGTTAATCCAGTCTGGTGCCAAA
ATTGAATGGGTCTTGGAACATCTTTCCCATGAACTTTCAGCTCCAATTCCTTTCTATAAG
CGTTCACTGCATCCTGTGTCATCCTTGA
   
Sequence Name 58-K102369-0022-625-G03C-NG70
GenBank Accession LN509665 [GenBank]
Sequencing primer gene specific primer, NG70: 5'-CAAGGATATCTCAAGGATGACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K102369-0022-625-G03C-NG70
GATTGGAGCTGAAGTTCATGGGAAAGATGTTCCAAGACCCATTCAATTTTGGCACCAGAC
TGGATTAACTAGCAAAATTTTGGATACTCTGAAGAAGCTTAACTATGAAAAGCCAATGCC
TATCCAAGCACAAGCACTTCCAATCATCATGAGCGGTAGAGACTGCATTGGGGTTGCCAA
AACCGGGTCAGGTAAAACCCTTGGCTTTGTTTTGCCTATGTTGAGGCATATCAAAGATCA
GCCTCCCGTTGAAGCTGGTGATGGGCCGATTGGGCTTGTAATGGCACCTACTAGAGAACT
TGTTCAGCAGATTTACAGCGATATCAGAAAGTTTTCTAAGGCATTGGGTATAATATGTGT
GCCTGTGTATGGAGGATCTGGAGTTGCCCAGCAAATCAGTGAGCTTAAGCGAGGTACCGA
GATTGTTGTTTGTACTCCTGGAAGAATGATCGATATTCTTTGCACAAGCAGTGGAAAAAT
TACCAATCTACGAAGGGTCACATATTTGGTAATGGATGAAGCTGATCGTATGTTTGATAT
GGGTTTTGACCTCTGTCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGAGGGTCAATAT

Last Updated on 06.12.2018 16:11