Sequences from confirmation amplicon


Confirmation sequences for line 625G10 and hit At5g46350 are shown.
Line ID 625G10
Locus At5g46350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18801800
Primers used for PCR IS57 - 8474, show primer details
   
Sequence Name 57-K023621-022-625-G10C-8409
GenBank Accession LN509290 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K023621-022-625-G10C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACAGGAGTTACTATAT
TTCAATTACATGAACAGGAGTTACTATAGATGCACGACTCAGAAGTGCAACGTGAAGAAG
AGAGTGGAGAGATCTTACCAAGACCCAACGGTCGTCATCACAACCTACGAGAGTCAACAC
AACCATCCGATCCCGACCAATCGTCGGACAGCAATGTTCTCTGGAACCACCGCATCTGAT
TATAACCCATCATCGTCTCCAATATTCTCCGATCTCATCATCAATACTCCAAGAAGCTTC
TCAAATGATGATCTCTTCCGTGTGCCATACGCTAGTGTGAACGTGAACCCTAGTTATCAT
CAACAGCAACATGGATTTCATCAACAGGAGAGTGAGTTCGAGCTCTTGAAGGAGATGTTT
CCTTCGGTTTTCTTCAAACAAGAGCCTTGATGATATAATATAATATAGAAACAATTTTTT
TTCTGCTAAGAAATATAGAACAAAACTTGGATGCATAATAAGTGATGATAGTGTTATTTA
TTTTTTGCATGTATATATTATACATGTTTTGTTAACTAGCTATAGGATATACTGTAGTAA
TTAAGCATAAATATGGAGTCCCTTCGACTTATTACAATAATTTTTGTTATGGAGTAATTT
TG
   
Sequence Name 58-K023621-022-625-G10C-IS57
GenBank Accession LN509585 [GenBank]
Sequencing primer gene specific primer, IS57: 5'-GCATGCATGTAACAAAATTACTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K023621-022-625-G10C-is57
ATAACAAAAATTATTGTAATAAGTCGAAGGGACTCCATATTTATGCTTAATTACTACAGT
ATATCCTATAGCTAGTTAACAAAACATGTATAATATATACATGCAAAAAATAAATAACAC
TATCATCACTTATTATGCATCCAAGTTTTGTTCTATATTTCTTAGCAGAAAAAAAATTGT
TTCTATATTATATTATATCATCAAGGCTCTTGTTTGAAGAAAACCGAAGGAAACATCTCC
TTCAAGAGCTCGAACTCACTCTCCTGTTGATGAAATCCATGTTGCTGTTGATGATAACTA
GGGTTCACGTTCACACTAGCGTATGGCACACGGAAGAGATCATCATTTGAGAAGCTTCTT
GGAGTATTGATGATGAGATCGGAGAATATTGGAGACGATGATGGGTTATAATCAGATGCG
GTGGTTCCAGAGAACATTGCTGTCCGACGATTGGTCGGGATCGGATGGTTGTGTTGACTC
TCGTAGGTTGTGATGACGACCGTTGGGTCTTGGTAAGATCTCTCCACTCTCTTCTTCACG
TTGCACTTCTGAGTCGTGCATCTATAGTAACTCCTGTTCATGTAATTGAAATATAGTAAC
TCCTGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGGAAATCTAC

Last Updated on 06.12.2018 16:11