Sequences from confirmation amplicon


Confirmation sequences for line 626C01 and hit At3g19130 are shown.
Line ID 626C01
Locus At3g19130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6613653
Primers used for PCR NL11 - 8474, show primer details
   
Sequence Name 04-K103979-0022-626-C01C-NL11
GenBank Accession LN487891 [GenBank]
Sequencing primer gene specific primer, NL11: 5'-GCCTCCTTTTAAGACCAAATCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103979-0022-626-C01C-NL11
AGCTCACGAGCTCTCACTTTCTTTACGCACACTTGTTGGAACGAAATATATTCATAGAGA
AGAAGAATCTCGAAACTCTTGAATCTAAAACAACTCGGAGACTCTGTTTTCCCTTTCCGG
AGATCAGATCGGATCAAGATGCAGACAACCAACGGCTCAGATTCGACGTTGGCAACTTCC
GGAGCCACACCGCCGAATCAACAAACCCCTCCTCCACCTCAGCAGTGGCAGCAGCAGCAA
CAGCAACAGCAACAGTGGATGGCTGCCATGCAATATCCACCAGCGGCGGCGATGATGATG
ATGCAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCG
   
Sequence Name 20-K103980-0022-626-C01C-8409
GenBank Accession LN495184 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K103980-0022-626-C01C-8409
GGACTGAGCCTTTACATTGAATATATCCTGCTGCATCATCATCATCGCCGCCGCTGGTGG
ATATTGCATGGCAGCCATCCACTGTTGCTGTTGCTGTTGCTGCTGCTGCCACTGCTGAGG
TGGAGGAGGGGTTTGTTGATTCGGCGGTGTGGCTCCGGAAGTTGCCAACGTCGAATCTGA
GCCGTTGGTTGTCTGCATCTTGATCCGATCTGATCTCCGGAAAGGGAAAACAGAGTCTCC
GAGTTGTTTTAGATTCAAGAGTTTCGAGATTCTTCTTCTCTATGAATATATTTCGTTCCA
ACAAGTGTGCGTAAAGAAAGTGAGAGCTCGTTGAGCTCTGGTAATGGCGGAGAGAGAGAT
AGAGGTAGAGAGAGATTTGGTCTTAAAAGGAGG

Last Updated on 06.12.2018 16:11