Sequences from confirmation amplicon


Confirmation sequences for line 626C07 and hit At3g18450 are shown.
Line ID 626C07
Locus At3g18450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6331483
Primers used for PCR MG17 - 8474, show primer details
   
Sequence Name 11-K031455-022-626-C07C-MG17
GenBank Accession LN490994 [GenBank]
Sequencing primer gene specific primer, MG17: 5'-CAACAAACACAAAATCAGTGGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K031455-022-626-C07C-MG17
AATGGTTAGATAACTTTCTAACACAACAAAAAAAACTAAACATTCTATATTCTACGATTG
GTTTCTCCATTTTTTGTTGTTCTTGTTGTTGTTGTGCATTGGATTAGCCGTGATCACATT
GATAGCTCCATGCGTCACGTTCGGACAGATTGCGGAGATCGTTGACGAAGGCGCGACGCG
TAAGTCATTCATTCATACACAAACCTTTATTAAGAAAAACACTCATAGATTGTAATGTCT
CATGTGTGTAAACATATTTCTATCACGCAGCTTGTGCGACAGCTGGGTTGTTGTACGGAG
CACTATTTTTTACCGGGGCAAGTTTTGTGTATTCATACATGTTCAGGGCCAGGATACGAA
AGAAGTTCGGGTTACCGGATGCTCCGGCTCCCGATTGGATCACTCACTTAGTCTGTATGC
CGTTTGCTCTTTGTCAAGAGTATCATGAATGAGTATCTTTGTCCGGCTCCCGATTGGATC
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATTCAC
   
Sequence Name 03-K031455-022-626-C07C-8409
GenBank Accession LN487362 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K031455-022-626-C07C-8409
CATTTACAATTGGATCCAATCGGGAGCCGGACAAAGATACTCATTCATGATACTCTTGAC
AAAGAGCAAACGGCATACAGACTAAGTGAGTGATCCAATCGGGAGCCGGAGCATCCGGTA
ACCCGAACTTCTTTCGTATCCTGGCCCTGAACATGTATGAATACACAAAACTTGCCCCGG
TAAAAAATAGTGCTCCGTACAACAACCCAGCTGTCGCACAAGCTGCGTGATAGAAATATG
TTTACACACATGAGACATTACAATCTATGAGTGTTTTTCTTAATAAAGGTTTGTGTATGA
ATGAATGACTTACGCGTCGCGCCTTCGTCAACGATCTCCGCAATCTGTCCGAACGTGACG
CATGGAGCTATCAATGTGATCACGGCTAATCCAATGCACAACAACAACAAGAACAACAAA
AAATGGAGAAACCAATCGTAGAATATAGAATGTTTAGTTTTTTTTGTTGTGTTAGAAAGT
TATCTAACCATTTTCACTGTCGTTCATGCAATCGAAAAGTTGAGAACTCCACTGATTTTN
GTGTTTGT

Last Updated on 06.12.2018 16:11