Sequences from confirmation amplicon


Confirmation sequences for line 626C12 and hit At2g01270 are shown.
Line ID 626C12
Locus At2g01270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:140151
Primers used for PCR 21wm - 8474, show primer details
   
Sequence Name 86-K118585-0022-626-C12C-21wm
GenBank Accession LN514699 [GenBank]
Sequencing primer gene specific primer, 21wm: 5'-TCTTCAGTTACTAGTTTGGGCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K118585-0022-626-C12C-21wm
TCCTGCCTGTAGAAACTACAGGTAACATAAACTATTCAAAGCCTCAACTTTGGAGATTTC
CGTTGCCTAAAAAGTGTTACTCTTATGCAGCCTCATTACGAAAAGGTTGCAAGGCTCTTC
AATGGTCCAGATGCAATACATCCAGGAATCGTTTTGATGACTAGAGTTGATTGTGCAATG
AAGGTATTTACTCCATGCTCCGAATGTTTTTATGAATCATGTTAAATTCTCTTGGATCGA
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTA
   
Sequence Name 56-K118584-0022-626-C12C-8409
GenBank Accession LN509242 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K118584-0022-626-C12C-8409
CATTTCATTGAATTATTCGATCCAGAGAATTTAACATGATTCATAAAAACATTCGGAGCA
TGGAGTAAATACCTTCATTGCACAATCAACTCTAGTCATCAAAACGATTCCTGGATGTAT
TGCATCTGGACCATTGAAGAGCCTTGCAACCTTTTCGTAATGAGGCTGCATAAGAGTAAC
ACTTTTTAGGCAACGGAAATCTCCAAAGTTGAGGCTTTGAATAGTTTATGTTACCTTGTA
GTTTCTACAGGCAGGACACCTAGAATCAATTGAAAACGATACACGCATCAGTAAACGCCC
AAACTAGTAACTGAAGAACC

Last Updated on 06.12.2018 16:11