Genotyping and primer details


Genotyping and primer details for 626C12 - At2g01270
Line ID 626C12
Locus At2g01270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:140151 of line 626C12 Chr2:140151
   
Insertion allele amplicon 8474 - 21wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21wm: 5'-TCTTCAGTTACTAGTTTGGGCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:139858
   
wt amplicon (over InsPos) 86ww - 21wm
wt amplicon start Chr2:139858
wt amplicon end Chr2:140969
wt amplicon size 1112 bp
Locus specific primer 21wm: 5'-TCTTCAGTTACTAGTTTGGGCGTT-3'
2nd locus specific primer 86ww: 5'-GACGGCATTAGGATAATGACACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11