Sequences from confirmation amplicon


Confirmation sequences for line 626D02 and hit At4g18950 are shown.
Line ID 626D02
Locus At4g18950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10377182
Primers used for PCR BF11 - 8474, show primer details
   
Sequence Name 55-K028749-022-626-D02C-8409
GenBank Accession LN508699 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K028749-022-626-D02C-8409
TAGTTTTACCAACGTCAGAACGAAAACAGAAAGAGTTTGCCGTCAGTAACCGCACATATA
AGAGACAGAAACCACCGATATGGACAGTGTGACTCACGAAGGTTCAAGATCGCGGTGGAT
TATAGGGTCTCCTTTGATCTCATGAAGATAGCTCATTCCCCTAGGATAATTCGAACGGGT
AAGATTTACAAGAGAATCTTATAGAACATATATAAACAACGAAGAAGAGACATATATAGC
TTCAACTTGCATACCTAGCAATATCAAGGGCATATCTAACAGCAGTAGCTGGTTTCAATT
GTCCTTTTCGTTTGAGCAATTCACGCAAATCCCCCTGAAGAATATATCAAATGAGATCTT
GGTTTCGTGAAACTAAATGCAAAAATAATTTCAAATTTATCAGAGAAAGTCAGTACCCTG
GGCAAATATTCAGTCACA
   
Sequence Name 55-K028749-022-626-D02C-BF11
GenBank Accession LN508700 [GenBank]
Sequencing primer gene specific primer, BF11: 5'-GTAACCCAATGATGATTGTGACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K028749-022-626-D02C-BF11
CATTTAGTTTCACGAAACCAAGATCTCATTTGATATATTCTTCGGGGGATTTGCGTGAAT
TGCTCAAACGAAAAGGACAATTGAAACCAGCTACTGCTGTTAGATATGCCCTTGATATTG
CTAGGTATGCAAGTTGAAGCTATATATGTCTCTTCTTCGTTGTTTATATATGTTCTATAA
GATTCTCTTGTAAATCTTACCCGTTCGAATTATCCTAGGGGAATGAGCTATCTTCATGAG
ATCAAAGGAGACCCTATAATCCACCGCGATCTTGAACCTTCGTGAGTCACACTGTCCATA
TCAGTGGTTTCTGTCTCTTATATGTGCAGTTACTGACAGCAAACTCTTTCTGTTTTCGTT
CTGACATTGGTAAAACTATATTTGCAGAAACATTCTGCGGGATGATTCAGGGCATCTGAA
AGTTGCAGACTTTGGAGTAAGCAAGCTTGTTACTGTTAAAGAAGACAAGCCTTTTACATG
TCAAGACATTTCTTGTAAGTACTTTTTCGCACCCAACTAGTTACTGTAAATTTTGAATGC
TTTAATTCTTGATCTGCTTGGTTAAAGAGTCTTCATTACTGGATGTGTTATAATAAAAAT
TTATCGTAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT

Last Updated on 06.12.2018 16:11