Genotyping and primer details


Genotyping and primer details for 626D02 - At4g18950
Line ID 626D02
Locus At4g18950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10377182 of line 626D02 Chr4:10377182
   
Insertion allele amplicon 8474 - BF11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BF11: 5'-GTAACCCAATGATGATTGTGACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10376729
   
wt amplicon (over InsPos) 01mf - BF11
wt amplicon start Chr4:10376729
wt amplicon end Chr4:10377835
wt amplicon size 1107 bp
Locus specific primer BF11: 5'-GTAACCCAATGATGATTGTGACTG-3'
2nd locus specific primer 01mf: 5'-GCATTCTTCTATCAACCTATGGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11