Sequences from confirmation amplicon


Confirmation sequences for line 626H02 and hit At2g17230 are shown.
Line ID 626H02
Locus At2g17230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7494969
Primers used for PCR 14vy - 8474, show primer details
   
Sequence Name 25-K118269-0022-626-H02C-14vy
GenBank Accession LN497655 [GenBank]
Sequencing primer gene specific primer, 14vy: 5'-TGGATTTCACTATTTCACTTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118269-0022-626-H02C-14vy
CTTACGCTTGGGTTGGTCATCGGGAAAACAATGTCCCGAGGTTTGTGCTTATCCATTTGC
TTTACCGGGTTACATGGGACACGGTGGTCCCGGAGAGCTCCGGCCACCGAACGGAGAGAC
TGGAGTAGATGGGATGGTTAGTGTGATTGGTCATGAGTTAGCTGAAGTTGTATCGAATCC
GTTGATTAATGCTTGGTACGCCGGAGAAGATCCGACGGCTCCGACGGAGATCGGTGATTT
GTGTGAGGGATTGTACGGAAGCGGCGGAGGAGGAGGGTATATTGGTCAAGTTATGAGAGA
TAGAGAAGGGAAGACTTTTAATATGAATGGTAAAGCGTTAATGATCCACATGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGGTTATTA
   
Sequence Name 10-K118268-0022-626-H02C-8409
GenBank Accession LN490863 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118268-0022-626-H02C-8409
GCCATTTACAATTGAATATATCATGTGGATCATTAACGCTTTACCATTCATATTAAAAGT
CTTCCCTTCTCTATCTCTCATAACTTGACCAATATACCCTCCTCCTCCGCCGCTTCCGTA
CAATCCCTCACACAAATCACCGATCTCCGTCGGAGCCGTCGGATCTTCTCCGGCGTACCA
AGCATTAATCAACGGATTCGATACAACTTCAGCTAACTCATGACCAATCACACTAACCAT
CCCATCTACTCCAGTCTCTCCGTTCGGTGGCCGGAGCTCTCCGGGACCACCGTGTCCCAT
GTAACCCGGTAAAGCAAATGGATAAGCACAAACCTCGGGACATTGTTTTCCCGATTGACC
AACCCAAGCGTAAGGCATAGTGTAACCAACCATCGACGGGAAAGTGAAATAGTGAAATCC

Last Updated on 06.12.2018 16:11