Sequences from confirmation amplicon


Confirmation sequences for line 626H08 and hit At1g17230 are shown.
Line ID 626H08
Locus At1g17230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5893901
Primers used for PCR EU22 - 8474, show primer details
   
Sequence Name 49-K103393-0022-626-H08C-EU22
GenBank Accession LN506807 [GenBank]
Sequencing primer gene specific primer, EU22: 5'-AATCATAGCGACCACTTCTCTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K103393-0022-626-H08C-EU22
TGGTGCGAACAGTGCAATCTTTAGGACAGAGACATTTCATGAACTGTTCTCTTATCATTT
GTATCTAGCCTCGCATCAAACATTTCAATGGTTGGAATCATGTTACGGATCGATCGTCGA
ACCCAATTGACTAGATCCCCTCCTTGCTCCAGCGGTTGAACTGGGGGCTTTCCTGTTATT
AGCTCCAGAAGCACAACTCCAAAGCTATAGATATCACATTTCTCTGTCACCTTCATCGTG
TAAGCATATTCTGAAACACAGCAAAACATCAATTAGATATGTTAGATAAGCGATTGTGGG
AAGAAAGAAAACTGTGATGTTTTACTCACCTGGAGCGATGTATCCNTAAGACCCAGCAAC
AGCTGACATGGACTTTGAGTAAGAGAGATCAATCAACTTAGCCNGGCCAAAATCTCCAAC
ATGAGCCTGGAACCGTTCATCAAGAAGAATGTTGTTCGATTTTATGTCGCGATGGACTAT
TTGAGGTCTGCAATCATGATGGAGATAACACAAACCCTCTGCAGCACCGAGGGCGATTCT
ATACCGAGCATTCCAATCCAACAAACAGTTTTTCTCACCTCGCTGTAGTTGTTCGCCGAG
GCTTCCTTTTGACATGTACTCATACNAGAGAAGATTTGAATTCTGGTGATAGCAGAAACC
ATATNGCTTCACAATATTCCGGTGCCTTATCTNTCCNAGAGTTGATATCTCAGCTCTNAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAATGT
   
Sequence Name 39-K103394-0022-626-H08C-8409
GenBank Accession LN503321 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103394-0022-626-H08C-8409
ATTTACATTGAATATATCCTAAGAGCTGAGATATCAACTCTTGGAAAGATAAGGCACCGG
AATATTGTGAAGCTATATGGTTTCTGCTATCACCAGAATTCAAATCTTCTCTTGTATGAG
TACATGTCAAAAGGAAGCCTCGGCGAACAACTACNGCGAGGTGAGAAAAACTGTTTGTTG
GATTGGAATGCTCGGTATAGAATCGCCCTCGGTGCTGCAGAGGGTTTGTGTTATCTCCAT
CATGATTGCAGACCTCAAATAGTCCATCGCGACATAAAATCGAACAACATTCTTCTTGAT
GAACGGTTCCNGGCTCATGTTGGAGATTTTGGCCTGGCTAAGTTGATTGATCTCTCTTAC
TCAAAGTCCATGTCAGCTGTTGCTGGGTCTTACGGATACATCGCTCCCGGTGAGTAAAAC
ATCACAGTTTTCTTTCTTCCCACAATCGCTNATCTNACANATCTNATTGATGTTTTGCTG
TGTTTCAGAATATGCTTACACGATGAAGGTGACAGAGAAATGTGATATCTATNGCTTTGG
AGTTGTGCTTCTGGAGCTAATAACAGGAAAGCCCCCNGTTCAACCGCTGGAGCAAGGAGG
GGATCTNGTCAATTGGGTTCGACGATNGATCCGTAACATGATTCCCACCATTGAAATGTT
TGATGCGAGGCTNGATACAAATGATNAGAGAACAGTTCAGGAAATGTCTCTGGTCCTAAA
GATGGCACTGTTCTGCACCAG

Last Updated on 06.12.2018 16:11