Sequences from confirmation amplicon


Confirmation sequences for line 626H08 and hit At2g19990 are shown.
Line ID 626H08
Locus At2g19990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8630102
Primers used for PCR 15vy - 8474, show primer details
   
Sequence Name 31-K118107-0022-626-H08C-15vy
GenBank Accession LN500335 [GenBank]
Sequencing primer gene specific primer, 15vy: 5'-TTTAAATCTCCCAACACGAGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118107-0022-626-H08C-15vy
TTTTCTTTCCTTTTCTAAAAATATTATTAATAAAAATTCAACTTTAAAGTTTGGCAAAAA
AAAAAACCTTAAAACCTAAAAGGTTTGGAATATGGAATAAAAGGGCTATATCCGGTATAA
AAAATTTTCCACATATTTGAAAAAGGTCCTATTTGGTCCAAGGGTTTTCCGGTAATCCAA
CCTAAAACGGCCCATGTTTCCTTTGGCTGTAAAGCAAATTGAAAAAAAAATTTTGTAAAA
TTTCTAAATCCAATAATAACCAAATCACCACCTTTAATATTTCAGGAAACCATTTCTCAC
TTTGATGGAAAAAAACCAAGGGGGCATTTATTTTTCCTTTTACCATTAAGGGACCCAAAA
GGTTATAGTAGAAATTTTTCTAAATCCACTTAATTTCCCAAATATGGAAACCTGGTTAAT
CCAGTAAAAAAAAACCCAACCCCAAAAATGGACTTTAACCAATGTATTCCCCCGAAGAAT
CTACAAGGGCTCCTTGCCCGGAAAATCTCCTGGGATCACCATTGAGTATAAGGGTCAATA
TGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTCCATTCCAAAATTGTAAATTCCCCCG
CCTATTTTT
   
Sequence Name 12-K118106-0022-626-H08C-8409
GenBank Accession LN491783 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118106-0022-626-H08C-8409
ATCCTCCGGGGAATACATCGGTCAACGTCCATATTAGTGATTGGATTTTATTATACTGAA
TCAACAAGATTACTATATATGGTAATTAATGTGATTTAAGAAAAATTCATACTATAACAT
TTTGTGTCCTTAAATGATAAAAGCATATATAAATGCACACTAGTTTTTTTACCATCAATG
TGAGATATGTTTCCATGATATATCAAAGTTGTTGATTTGATTATAATCGACTTCAGAATT
CTTACAAATTTTTTCTTTCATTTCGCTCTACAGCAAAAGAAAACATGGACAGTTTAAGAT
TGAATAACAGAAAAACCCTAGAACAAAATAGAACATTTTCAAAATATGTGAAATATCTTT
AATACAGTATATAGCACTTTAATTCAATATACAAAACATATAAGATTTAAAGTTTTTTTT
TTTTGCCAAACTTTAAAGTTAAATTTTTATAAAAAATATTTTTAAAAAAGGAAAAAAAAA
ATTTTCCTTTTAAAAACGTTTTCAAAATTTAAATCTCGGGTGGGAAAAATTAAAA

Last Updated on 06.12.2018 16:11