Sequences from confirmation amplicon


Confirmation sequences for line 627A02 and hit At1g09550 are shown.
Line ID 627A02
Locus At1g09550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3091894
Primers used for PCR 23kq - 8474, show primer details
   
Sequence Name 67-K113756-0022-627-A02C-23kq
GenBank Accession LN511858 [GenBank]
Sequencing primer gene specific primer, 23kq: 5'-CATTAAGAGGAGAAATGAAAACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113756-0022-627-A02C-23kq
TTCTCTTCAGCATTCTTGCACATGGGGAAATGAAAACCTTCAACGATTCCAATGGAACAA
ATGCAAATGTCCTGATGGTAGGACTCACGCTCGTCCAAGCCGCTGCTGCTAAAGGAGCGG
GTAACAATTTTCTTACCATTCATTTCGATTGAGATTTAGATTTCTGATATGCATGTTAGA
TCTCCAATGTTTCAAACGTTAGTTTTCAAGTTTTTTTTACGAAATTTCGTTTGTACCGTG
TCATTATTTTTCATCATTATTACAAAGTTTTTGATTGTCATTTTCATGACGCATGGAGAA
ATAATTAAATTTTTGTGGCTTCATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCACAGTTATTAT
   
Sequence Name 29-K113755-0022-627-A02C-8409
GenBank Accession LN499374 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K113755-0022-627-A02C-8409
AGCCCAAAAATTTAATTATTTCTCCATGCGTCATGAAAATGACAACTCATAAACTTTGTA
ATAATGATGAAAAATAATGACACGGTACAAACGAAATTTCGTAAAAAAAACTTGAAAACT
AACGTTTGAAACATTGGAGATCTAACATGCATATCAGAAATCTAAATCTCAATCGAAATG
AATGGTAAGAAAATTGTTACCCGCTCCTTTAGCAGCAGCGGCTTGGACGAGCGTGAGTCC
TACCATCAGGACATTTGCATTTGTTCCATTGGACTCGTTGAAGGTTTTCATTTCCCCATG
TGCAAGAATGCTGAAGAGAATGAGTCCTGCGAGACTACTCCAACCCCAATACAAAAGCGT
TTTCATTTCTCNTCTTAATG

Last Updated on 06.12.2018 16:11