Sequences from confirmation amplicon


Confirmation sequences for line 627C01 and hit At3g55090 are shown.
Line ID 627C01
Locus At3g55090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20416917
Primers used for PCR MZ68 - 8474, show primer details
   
Sequence Name 51-K026771-022-627-C01C-8409
GenBank Accession LN507379 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K026771-022-627-C01C-8409
GTAGATTTCCCGGACATGAAGCCATTTACTCACTGCGAAAGCTAAAGAGAGGAAGATGAG
CGAAGGGAAGGTAACAATGGCGTGAGAGAGAACGTAGGAGGATCTCCGGTAAGCGTTGTA
AGCTGTTTCTCTCATGAAGATGTAACGTTCCTGGAGGAACACGGGAAGAGCATCGGCGCA
AGTGTAGAACATTGTTGACATTGCGAATGCGAAGAACCCTAACCGCTCTTGAACTCCTTT
TGGTGAATTATCCAATCGCCAGAATACGGTGGCTAAGATGAATCCGGTGACGATTACGGT
GGCTAATCGCATTCCGAGTAGCTCCGGTTGTCGTCGGGAGTTGAGGATTGAGCGTCTGGT
GAGAGTTTTGATTTCGATCCAGAACGGATTCGCGAATGCAGGGACGGCTAAGGTTCCGCC
ACCGTGGTTTATGACGGAGGAACCACCGCCGCCACCGGAGACTAGTTTTCCTCTGCTTAT
GCTGGCGGAAATCGCTTCTTTCAAGGTTAAATTTGGGTTTGGAGAAGCCGGCGGAGTTAG
GGTTTGAGGATTGCTCTGTTTCTTCATCTCTTGCCATTTCTTGTTGAATTCAACTAA
   
Sequence Name 52-K026771-022-627-C01C-MZ68
GenBank Accession LN507693 [GenBank]
Sequencing primer gene specific primer, MZ68: 5'-CTGGAGGAACAAGAGGATTAGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K026771-022-627-C01C-mz68
AACAAGAAATGGCAAGAGATGAAGAAACAGAGCAATCCTCAAACCCTAACTCCGCCGGCT
TCTCCAAACCCAAATTTAACCTTGAAAGAAGCGATTTCCGCCAGCATAAGCAGAGGAAAA
CTAGTCTCCGGTGGCGGCGGTGGTTCCTCCGTCATAAACCACGGTGGCGGAACCTTAGCC
GTCCCTGCATTCGCGAATCCGTTCTGGATCGAAATCAAAACTCTCACCAGACGCTCAATC
CTCAACTCCCGACGACAACCGGAGCTACTCGGAATGCGATTAGCCACCGTAATCGTCACC
GGATTCATCTTAGCCACCGTATTCTGGCGATTGGATAATTCACCAAAAGGAGTTCAAGAG
CGGTTAGGGTTCTTCGCATTCGCAATGTCAACAATGTTCTACACTTGCGCCGATGCTCTT
CCCGTGTTCCTCCAGGAACGTTACATCTTCATGAGAGAAACAGCTTACAACGCTTACCGG
AGATCCTCCTACGTTCTCTCTCACGCCATTGTTACCTTCCCTTCGCTCATCTTCCTCTCT
TTAGCTTTCGCAGTGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11