Sequences from confirmation amplicon


Confirmation sequences for line 627C03 and hit At5g66670 are shown.
Line ID 627C03
Locus At5g66670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26611966
Primers used for PCR CH87 - 8474, show primer details
   
Sequence Name 13-K107031-0022-627-C03C-8409
GenBank Accession LN492062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K107031-0022-627-C03C-8409
TCGGACTGAGCATTTACAATTGAATATTCCTCAATCAATTGAATATTTCTCCATATTGTC
GTTTTAAACGCAGTGGCGAAAACCACATTTGACAATATCCTCCATGTCTTTATATTCCTC
AGTTTCTTACCAAGCTTTACTTTCAGCTTACGCAACTCGTCCAGAAGCAAAACCTGCTCA
TCGTAGACAGACTTGTACTGAGTCACGAACTCGCCATCAAAAGGGTCTCCCATGGCTTTA
AACTTGTTCATCTCCTCCAAGGTTTTCACATACTTCTTCTTCTTACTCTCTCCAAGATCC
GTATCCACCGTTTCTGTCTCAAACTGTTTCACCGCGAATCGAATGATAAGCTGGCTTATT
TCTGCTTTCTTGACGCATTTCTCGACGGTATTGCAGAAGTCCAAGGTCTTAGAGGTACTT
TTGAAATAGACATCAACCAAAGCCTTGAGATCATTGTTCTTCAAAACATCTTCTTTGCTT
TCAATGATGA
   
Sequence Name 29-K107032-0022-627-C03C-CH87
GenBank Accession LN499311 [GenBank]
Sequencing primer gene specific primer, CH87: 5'-TCATCATTGAAAGCAAAGAAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K107032-0022-627-C03C-CH87
AGGCTTTGGTTGATGTCTATTTCAAAGTACCTCTAAGACCTTGGACTTCTGCAATACCGT
CGAGAAATGCGTCAAGAAAGCAGAAATAAGCCAGCTTATCATTCGATTCGCGGTGAAACA
GTTTGAGACAGAAACGGTGGATACGGATCTTGGAGAGAGTAAGAAGAAGAAGTATGTGAA
AACCTTGGAGGAGATGAACAAGTTTAAAGCCATGGGAGACCCTTTTGATGGCGAGTTCGT
GACTCAGTACAAGTCTGTCTACGATGAGCAGGTTTTGCTTCTGGACGAGTTGCGTAAGCT
GAAAGTAAAGCTTGGTAAGAAACTGAGGAATATAAAGACATGGAGGATATTGTCAAATGT
GGTTTTCGCCACTGCGTTTAAAACGACAATATGGAGAAATATTCAATTGATTGAGGAATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTA

Last Updated on 06.12.2018 16:11