Sequences from confirmation amplicon


Confirmation sequences for line 627D04 and hit At1g04110 are shown.
Line ID 627D04
Locus At1g04110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1063644
Primers used for PCR TK04 - 8474, show primer details
   
Sequence Name 25-K027364-022-627-D04C-8409
GenBank Accession LN497294 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K027364-022-627-D04C-8409
GCCATTTACAATTGAATATATCCTGGTTTGCAAGCTCAGTTATATTGTACATTTTTGGCA
TCTTTCTTTTCTCCAAGAAGCGGTTTTAGGTGTTGAAGAAGAAGAGGAAGAGCCTTCTTC
TCGACTTCTCTACTCCTATGGCTCTGCGATTGAAGGATTTGCTGCTCAGTTGACTGAATC
AGAAGCCGAGATACTGAGATATTCACCTGAAGTTGTTGCAGTGAGACCTGACCATGTTCT
TCAGGTTCAAACCACTTACTCTTACAAGTTCTTGGGACTCGACGGTTTTGGAAACTCCGG
TGTATGGTCTAAATCTCGGTTTGGTCAAGGCACAATTATCGGCGTGCTTGATACTGGAGT
TTGGCCTGAAAGTCCTAGCTTTGACGATACCGGAATGCCTTCGATTCCACGGAAATGGAA
AGGGATTTGCCAAGAAGGAGAAAGTTTCAGTTCTTCGAGCTGTAACCGGAAGCTAATCGG
TGCTAGATTCTTCATCAGAGGACACCGTGTCGCTAATTCACCAGAGGAATCACCAAACAT
GCCTCGTGAATACATTTCCGCAAGAGATTCAACGGGACACGGGACTCACACCGCCTCAAC
AGTTGGTGGATCCTCTGTTTCGATGGCGAATGTTCTTGGCAATGGAGCTGGTGTGGCTCG
TGGGATGGCTCCTGGAGCTCACATTGCAGTCTATAAAGTCTGTTGGTTCAATGGT
   
Sequence Name 26-K027364-022-627-D04C-TK04
GenBank Accession LN497768 [GenBank]
Sequencing primer gene specific primer, TK04: 5'-ATGTCAGAGCTGTAACAACCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K027364-022-627-D04C-TK04
TGAGCTCCGGAGCCATCCCACGAGCCACACCAGCTCCATTGCCAAGAACATTCGCCATCG
AAACAGAGGATCCACCAACTGTTGAGGCGGTGTGAGTCCCGTGTCCCGTTGAATCTCTTG
CGGAAATGTATTCACGAGGCATGTTTGGTGATTCCTCTGGTGAATTAGCGACACGGTGTC
CTCTGATGAAGAATCTAGCACCGATTAGCTTCCGGTTACAGCTCGAAGAACTGAAACTTT
CTCCTTCTTGGCAAATCCCTTTCCATTTCCGTGGAATCGAAGGCATTCCGGTATCGTCAA
AGCTAGGACTTTCAGGCCAAACTCCAGTATCAAGCACGCCGATAATTGTGCCTTGACCAA
ACCGAGATTTAGACCATACACCGGAGTTTCCAAAACCGTCGAGTCCCAAGAACTTGTAAG
AGTAAGTGGTTTGAACCTGAAGAACATGGTCAGGTCTCACTGCAACAACTTCAGGTGAAT
ATCTCAGTATCTCGGCTTCTGATTCAGTCAACTGAGCAGCAAATCCTTCAATCGCAGAGC
CATAGGAGTAGAGAAGTCGAGAAGAAGGCTCTTCCTCTTCTTCTTCAACACCTAAAACCG
CTTCTTGGAGAAAAGAAAGATGCCAAAAATGTACAATATAACTGAGCTTGCAAACAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC

Last Updated on 06.12.2018 16:11