Genotyping and primer details

Genotyping and primer details for 627D04 - At1g04110
Line ID 627D04
Locus At1g04110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1063644 of line 627D04 Chr1:1063644
Insertion allele amplicon 8474 - TK04
Locus specific primer TK04: 5'-ATGTCAGAGCTGTAACAACCATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1062967
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11