Sequences from confirmation amplicon


Confirmation sequences for line 629D02 and hit At1g11460 are shown.
Line ID 629D02
Locus At1g11460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3858254
Primers used for PCR 77sa - 8474, show primer details
   
Sequence Name 47-K116321-0022-629-D02C-77sa
GenBank Accession LN506307 [GenBank]
Sequencing primer gene specific primer, 77sa: 5'-ATATACGATGACACCGATCACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K116321-0022-629-D02C-77sa
GACTCAATAGAGCACATTGAAAAGCTGCGAAAATTGACATGAGACAAGTGCTTGAGAATT
TGCAAGGGTACTTGATGCTTAAAGTCCCTTGAAACAAAATCCATAGAGATATCAACACGG
TTCCTATGGTTAAGTAGAGACATCCAAGAAGCCAATTCTTTGTGTTGTGATCGCTGTTGT
TGTTGTGAGGAAGAGCCTCTTGGTGAGAGTGAGAGTTTGATATATGAGGGCCTTTGTAAA
ATGTTAAGAACAAAGCTCCACTTATACATATAATAGTTCCCATCACTTTTATCATTCCTG
CTTGGGACATATCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 15-K116320-0022-629-D02C-8409
GenBank Accession LN493134 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116320-0022-629-D02C-8409
CAGGAATGATAAAAGTGATGGGAACTATTATATGTATAAGTGGAGCTTTGTTCTTAACAT
TTTACAAAGGCCCTCATATATCAAACTCTCACTCTCACCAAGAGGCTCTTCCTCACAACA
ACAACAGCGATCACAACACAAAGAATTGGCTTCTTGGATGTCTCTACTTAACCATAGGAA
CCGTGTTGATATCTCTATGGATTTTGTTTCAAGGGACTTTAAGCATCAAGTACCCTTGCA
AATTCTCAAGCACTTGTCTCATGTCAATTTTCGCAGCTTTTCAATGTGCTCTATTGAGTC
TTTACAAGAGTAGAGATGTTAAAGATTGGATCATCGATGATAGATTTGTGATCGG

Last Updated on 06.12.2018 16:11