Sequences from confirmation amplicon


Confirmation sequences for line 629D03 and hit At3g22220 are shown.
Line ID 629D03
Locus At3g22220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7841770
Primers used for PCR 28lx - 8474, show primer details
   
Sequence Name 31-K114217-0022-629-D03C-28lx
GenBank Accession LN500256 [GenBank]
Sequencing primer gene specific primer, 28lx: 5'-CTGTCGAAAGATGTTTAAAGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114217-0022-629-D03C-28lx
CACTATCTGTGATCAGTTCCTGATGAGGTTCGTCTTTTCTTGCAGCAGTGTATTGATGGT
ACGGTGAGACGTCAGAGGAAGAGACGTAAATCAAGTCCTGAACCATTGCCTATAGCTTAT
TTCCCTCCTTGTGAAGTTGAGACTCAGGTTGCTGCTTCTTCTGATGTGAATAACGGGTTT
AAATCGCCGAGTTCGGATGTCGTAGTGGGTCAAAGTACTGGGAGGACGAAGCAGAGGACT
TATAGGAGTAGAAAGAATAATGCTTTTGAGAGGAATGATTTGGCTAATGTTGAGGTTGAT
CGAGATATGGACAATTTGATTCCTGTGGCTATTAGTAGCGTGAAGAACATTGTGCATCCT
ACTTCGAAAGAGCGGGAGAAAACAGTTCATATGGCTATGGGACGGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 13-K114216-0022-629-D03C-8409
GenBank Accession LN492133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114216-0022-629-D03C-8409
CATTTACCGTCCATAGCCATATGAACTGTTTTCTCCCGCTCTTTCGAAGTAGGATGCACA
ATGTTCTTCACGCTACTAATAGCCACAGGAATCAAATTGTCCATATCTCGATCAACCTCA
ACATTAGCCAAATCATTCCTCTCAAAAGCATTATTCTTTCTACTCCTATAAGTCCTCTGC
TTCGTCCTCCCAGTACTTTGACCCACTACGACATCCGAACTCGGCGATTTAAACCCGTTA
TTCACATCAGAAGAAGCAGCAACCTGAGTCTCAACTTCACAAGGAGGGAAATAAGCTATA
GGCAATGGTTCAGGACTTGATTTACGTCTCTTCCTCTGACGTCTCACCGTACCATCAATA
CACTGCTGCAAGAAAAGACGAACCTCATCAGGAACTTGATCACAGATAGTGCCTTGTCCT
TTCTTGCCAGCAAGATGTTCCTTAACCCTTGTAATACCACCTCCTTTAAACTCTNNTCGA
CA

Last Updated on 06.12.2018 16:11