Sequences from confirmation amplicon


Confirmation sequences for line 630H03 and hit F12M16 are shown.
Line ID 630H03
Locus F12M16 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19888732
Primers used for PCR 05wq - 8474, show primer details
   
Sequence Name 69-K118544-0022-630-H03C-05wq
GenBank Accession LN512285 [GenBank]
Sequencing primer gene specific primer, 05wq: 5'-TCGTAGACTGCCGATTTTAGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118544-0022-630-H03C-05wq
AGATGATGGTCGGTTAATACTTAAGTCCTTTATAAGAAAGAAAAATTAAAAGTCAACTTT
ATCGCTGAAAGTGAAAGTTAGGTTGAAAGTTCAGAGATTTATTCATCAACTTCCGTGCAT
TGACACATGCTCTTCTCTACATTCGCTCACGTGAATTTTAGAACAACAAATTATTTTACA
AACACTATAATCTTCCGGTAATTTAACCACAATAAAGAGATAAATAATCTTTATATTAAA
TCGATCGAATTCTAATTCTGTAGGTACTGTGAGTCAAACATGAAAGTGTAAATTTTCAAA
ATATGATTGGTGTTAACTTAGGTGAATCAATTTTTGATGTTTTTTTGTTGATTATATAAA
TCAAAATTACGGAAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGT
   
Sequence Name 49-K118543-0022-630-H03C-8409
GenBank Accession LN506991 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118543-0022-630-H03C-8409
GTAATTTTGATTATATAATCAACAAAAAAACATCAAAAATTGATTCACCTAAGTTAACAC
CAATCATATTTTGAAAATTTACACTTTCATGTTTGACTCACAGTACCTACAGAATTAGAA
TTCGATCGATTTAATATAAAGATTATTTATCTCTTTATTGTGGTTAAATTACCGGAAGAT
TATAGTGTTTGTAAAATAATTTGTTGTTCTAAAATTCACGTGAGCGAATGTAGAGAAGAG
CATGTGTCAATGCACGGAAGTTGATGAATAAATCTCTGAACTTTCAACCTAACTTTCACT
TTCAGCGATAAAGTTGACTTTTAATTTTTCTTTCTTATAAAGGACTTAAGTATTTAACCG
ACCATCATCTATTTCTTTCGGTTTTATTAGTTCTTCTAACCTCCCAAAATCTAAAA

Last Updated on 06.12.2018 16:11