SimpleSearch - Line and FST details
Line specific information
| Line ID | 630H03 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N460471) |
| Segregation Analysis | 50:15:10 |
| Confirmed for Hit | F12M16 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 1224, 4428, 4490, 10041, 87654, 87662, 87663 |
| Note |
Gene hit F12M16
| Sequence (A. th genome BLAST matches underlined) | >49-K118543-0022-630-H03C-8409 TCTTTCGTAATTTTGATTATATAATCAACAAAAAAACATCAAAAATTGATTCACCTAAGT TAACACCAATCATATTTTGAAAATTTACACTTTCATGTTTGACTCACAGTACCTACAGAA TTAGAATTCGATCGATTTAATATAAAGATTATTTATCTCTTTATTGTGGTTAAATTACCG GAAGATTATAGTGTTTGTAAAATAATTTGTTGTTCTAAAATTCACGTGAGCGAATGTAGA GAAGAGCATGTGTCAATGCACGGAAGTTGATGAATAAATCTCTGAACTTTCAACCTAACT TTCACTTTCAGCGATAAAGTTGACTTTTAATTTTTCTTTCTTATAAAGGACTTAAGTATT TAACCGACCATCATCTATTTCTTTCGGTTTTATTAGTTCTTCTAACCTCCCAAAATCTAA AATCG |
| GenBank Accession | HG968007 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:19888726 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | F12M16 |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At5g20930
| Sequence (A. th genome BLAST matches underlined) | >24-K114693-0022-630-H03-8409 GTTTATAAAACAGGAAGACTTAAAATCTTTGCGTGCAAAAATCGCTATGCTGGAGGAGGA ATTGCGTAAATCTCGACAAGATTCTTCTGAGTATCACCACCTTGTCAGGAATCTTGAGAA TGTATTGTGTGAATGCCTTTCAAATTTAGCTATTTGCGCCTATACTGAGTGCTATTACCA TGGACCCGCCTGCCCGGCCTATATATATAAACCCGGGGGGGAGTTATATAATCATGGNGC GGGNNNNTATTAANTTTTATNANNNNNNNNNNNGGGGGGNGNTTNNNNNNGNNNNNNNNG GGGGNNNNGGNNNNNNNNNNNNNNNNGNGNNNNNNGNNNNNAAANNGNNGGTNNNNNNNG ANNNNAAAAAAANNNNNNNNNNNNNNANNNNNAANNNNNTNNGNNNTGNTTTANTNTATA NNNATNTGNTTNNNTNTNNNTTAAAATTNNAAGGGNGAAAGNATAATTTATNTNNNTTGT ANNNTNTTTAATAATAGTAAAGATTGANCTAATAAANTATNNTAATATTGATNNNTTTAT NATCTTAATAATAGAAGTATATGTTTTGTTTGNCGAGGAGTTAAAAATATGCAATTCTTA ATTGTTACTTGTTGATGCTTTGTATGGTTTATTTGCAATATACTTGCTAGATTATCTTTG ATATGATGNTTGAGGGTATTATGATATTGAACTATTTGTATTTTGTAATGTATTATTTTA TTTGATATTATGTTCACTTTGTGTATTTATATTACNTATTATATGGAGTATTATTTATAA TCATATGATGCTTTTTGTCTTTCTATCTTACGTTGTTATATATGGTATTCTGTTTCTTTA TTAGGTTTTTTCTATTCTTTTATATATATTTCTTTCTAATTTATATCGTTTTTAGTTATT TACTCATTTTTTTAATTCTGTTATTTATATGTACTCTTATATTCTGTTATGTTTTTATAT TATTCTTATTGGTCTTTTTATGCTCGTATGTATTTTTATTTATTATGATTATAATGGAGT ATGTTCTTAGGCAGATTTCTTTATGTGTTGATAGTCGTATCGGGTATCGTTTTTTTTTAT GATCTTAATTCTTTCTTTGTGTTGCGTCTTCTTGATATTGTATTTTGTTGTTTGTCATTT GTAATGTTTTNTCTTGTAATATGTATATAATCATTAATTACTTATCTCCTTTATAATGTT TGCATGTGTCTAATTGTATATATATGTATATAATT |
| GenBank Accession | HE664964 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:7099588 - go to primer design |
| BLAST e Value | 1e-79 |
| Hit Clone Code (BAC ID) | F22D1 |
| Hit Gene Code | At5g20930 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Protein kinase superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
Gene hit At2g32680
| Sequence (A. th genome BLAST matches underlined) | >24-K114692-0022-630-H03-8409 TTCGGCCAGTTTAAGTTCCCATTCACACATCCCGCTGACCCAGGAAATGTTGACCTTGAG GCACTGTGACATCAACGATTTCCCCAACTACTTAAAGACCCTGAAGGAGTTGGTGTAGAT AGACATATCCGACAACGGAAAGAAAGGGAAGATCCCTGATTGGTTATGGAGCCTACCTCT TCTACAATCAATAACTCTTGGAAAAAATTATTTCGCATGTTTCCAAGGGCCGGCGGAGAT TTTATTAAATTCATCAGTGCTGCTATTATATTTGGATTCAAACGATTTTGAAGGAGCACT TCCCGATCTACCACTCTCTAATCAACGCTTCGGTGTGGCAAACCCGCC |
| GenBank Accession | HE664963 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:13861553 - go to primer design |
| BLAST e Value | 4e-125 |
| Hit Clone Code (BAC ID) | F24L7 |
| Hit Gene Code | At2g32680 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | receptor like protein 23 |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At5g35590
| Sequence (A. th genome BLAST matches underlined) | >24-K022050-022-630-H03-8409 AGCCATTTAAATTGAATATATCCTGGATAGTTAATAATTTTCTTGTAACTAAGGGAGCCC CCATATACTGAGAGACTCATGTGTAACCTGTATGTAGTCATGTCATTGAATAGATACACA AAACGTGTTTTGTTTCAACCAATGGGATCCTCCCTATAGTGAGAANAAAANNAAANANTA ATTACCAATTTTTTTTCAATTCAAAAATGTATATGTCCACAACGTTATTATA |
| GenBank Accession | BX892770 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:13766026 - go to primer design |
| BLAST e Value | 1e-30 |
| Hit Clone Code (BAC ID) | K2K18 |
| Hit Gene Code | At5g35590 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | proteasome alpha subunit A1 |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
|
Last Updated on 10.06.2021 13:37 |
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