Sequences from confirmation amplicon


Confirmation sequences for line 631C08 and hit At3g59100 are shown.
Line ID 631C08
Locus At3g59100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21848507
Primers used for PCR CS12 - 8474, show primer details
   
Sequence Name 24-K030755-022-631-C08C-8409
GenBank Accession LN496881 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K030755-022-631-C08C-8409
GCCATTTACATTGAATATATCCTGACAAGCAAGCAAGAAAGGTGGCCACCTGGATGACAG
ACACATCACCCGACGAAGATGGTACAAGCAGCAAATCCCTATCTCTGAGTTACAGCATAT
AAAGGAAACGTTACATGACATATATGCTTGCTAAACAGAACAGGACAATGATGACAAGAT
TGCTTTCTAAAAGAAAAGAATCTAACCTGTCACTGATCTTATCCTCGCTTCTCATAGAGT
AAATGAACTCGTTCCACACCTGGGAGAAATTAGTGATGTTTTTCTGGTCCACATAGTCAT
CCTACATTTCCCAGTGGGAATCAATAAATGACAGAACAGGGAATGAAGGTGTGATCCACC
TTTTGAGATATGGAAGGACAATAAACTATAAAAAGCACAGTACCGCATGTTTTCTTTTTG
CATCTTCGGATGGCATGAGGGTGCGGCTGAAGGCAATTGGTATTGACTCAAATCTAGATC
GCAACATCCCAAGAGTCCGTATCTATGAAA
   
Sequence Name 63-K030755-022-631-C08C-CS12
GenBank Accession LN510904 [GenBank]
Sequencing primer gene specific primer, CS12: 5'-GCTTTCATAGATACGGACTCTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K030755-022-631-C08C-CS12
ATACCAATTGCCTTCAGCCGCACCCTCATGCCATCCGAAGATGCAAAAAGAAAACATGCG
GTACTGTGCTTTTTATAGTTTATTGTCCTTCCATATCTCAAAAGGTGGATCACACCTTCA
TTCCCTGTTCTGTCATTTATTGATTCCCACTGGGAAATGTAGGATGACTATGTGGACCAG
AAAAACATCACTAATTTCTCCCAGGTGTGGAACGAGTTCATTTACTCTATGAGAAGCGAG
GATAAGATCAGTGACAGGTTAGATTCTTTTCTTTTAGAAAGCAATCTTGTCATCATTGTC
CTGTTCTGTTTAGCAAGCATATATGTCATGTAACGTTTCCTTTATATGCTGTAACTCAGA
GATAGGGATTTGCTGCTTGTACCATCTTCGTCGGGTGATGTGTCTGTCATCCAGTGGCCA
CCTTTCTTGCTTGCTTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11