Sequences from confirmation amplicon


Confirmation sequences for line 631E03 and hit At2g47320 are shown.
Line ID 631E03
Locus At2g47320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19428804
Primers used for PCR NK62 - 8474, show primer details
   
Sequence Name 04-K103431-0022-631-E03C-NK62
GenBank Accession LN487880 [GenBank]
Sequencing primer gene specific primer, NK62: 5'-AACCAAGCTTATCCAAACAAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103431-0022-631-E03C-NK62
TTGGTGGAGACTGGGTAGAACTCAGAGTCAAGCTCCCCCAGTAGCACCTACTCAAAACGC
CTCATCACACAACTCCAGCCAGAATCACTCACAGAGGTTTGATTCCTTTTAGCTTCACTA
TTTTGTTTAAGTTGTAACATATATAGTAACAGTATTGACCTGTTTCTCTTACTGTTGCAG
TTCTGAGAATCGGCCTAGAGAGAGGAAGAGAAGATCTGGTTGGGATAATTGAGTGAGCCA
CGTTTTGCATGATGATAGTGGAAGTGTTTTGCACAACAATGTAATGAATGTTGTATAATT
TGTTTTATCCTTTCCCGTTTCAGCCTTTGAATATATAAATTTTCCATTTCTTGTTAGGAT
CTAATTCTTCGTATGAGACATGATATGGAAAATAGATGATAAAAAGGGTTTCAAATTCAC
AAGAAAAATCACTTGAGTTTCATACAAGAGAGAGAGACAAGAAGTAAAAAAGCTTTTACA
TGTCTTGAAGCAAAGTGACGGACATGATCTCGCCAATAGAGACGTTGTTCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 20-K103432-0022-631-E03C-8409
GenBank Accession LN495175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K103432-0022-631-E03C-8409
ATTTACAATTGAATATATCCTGAACAACGTCTCTATTGGCGAGATCATGTCCGTCACTTT
GCTTCAAGACATGTAAAAGCTTTTTTACTTCTTGTCTCTCTCTCTTGTATGAAACTCAAG
TGATTTTTCTTGTGAATTTGAAACCCTTTTTATCATCTATTTTCCATATCATGTCTCATA
CGAAGAATTAGATCCTAACAAGAAATGGAAAATTTATATATTCAAAGGCTGAAACGGGAA
AGGATAAAACAAATTATACAACATTCATTACATTGTTGTGCAAAACACTTCCACTATCAT
CATGCAAAACGTGGCTCACTCAATTATCCCAACCAGATCTTCTCTTCCTCTCTCTAGGCC
GATTCTCAGAACTGCAACAGTAAGAGAAACAGGTCAATACTGTTACTATATATGTTACAA
CTTAAACAAAATAGTGAAGCTAAAAGGAATCAAACCTCTGTGAGTGATTCTGGCTGGAGT
TGTGTGATGAGGCGTTTTGAGTAGGTGCTACTGGGGGAGCTTGACTCTGAGTTCTACCCA
TGTCTCCACCAATAGTACCACCTGAGACAAAGCCCATTAGGGATGGTCTCTTGTTTG

Last Updated on 06.12.2018 16:11