Sequences from confirmation amplicon


Confirmation sequences for line 632B09 and hit At5g09970 are shown.
Line ID 632B09
Locus At5g09970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3112608
Primers used for PCR CP41 - 8474, show primer details
   
Sequence Name 63-K031140-022-632-B09C-CP41
GenBank Accession LN510906 [GenBank]
Sequencing primer gene specific primer, CP41: 5'-AACTTCTCCATTGTTCTTCTCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K031140-022-632-B09C-CP41
CCTTCTCAAGGATTTTTTTGCTTTCTCTTGAAAAACCTTTTGTTATTGTTATTGGTTCGT
ACGTTGTTCATTATTTGACAAGAAACAAGATTACAAAAAAAAAAAGTACTTCACCCCAAA
ATAAGGGATTTGATGAATTTGGCTTCAAAAAAAACAAGCTTTTGGATGATTGCCCTGCCG
GCCGGTTTTGGATCCCAAAACCTACATGATGTTTCCCCCCTAGGCTATCTTTTCCTTGCC
GTCGTTTTTCTCTCTATAGTCACGGGGGCTCTCCCCGGAGGCGGTGGTGTCCCTGGAAAA
AACGGCCGTAACCGGTGGGGTCGGGTCGCGATCCCGGGTCCTCGGGGCATACCATTATTC
GGCAGTCTTTTCACTCTCACCCAAGGCTTGGCTCATCGAACGTTAGCACCCTTGGCTTGG
ACCCAACCCAACACTGAAATTATGGCTTTTACCCTTGGTTCAACCCCGGTTATCGGGGCT
TCTGAACCAAATTGTAAATGGCTTCTTGTCCGGGAAATCTACTTGGATCACCAATGAGTA
TGATGGTCAATATGGAAAAAAAGAAAGAGTATTTCCCATTTTTTTTTCAATTCAAAAATG
TAAATGTCCCCACCGTTTTTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATGGGCAAAAGCAATAAACAAATTATTCTAATTCGAAAATCTTTATTT
CAACGTGTCTACTTTCCGTCCCAAAAAGGGGGA
   
Sequence Name 28-K031140-022-632-B09C-8409
GenBank Accession LN498722 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K031140-022-632-B09C-8409
ATTTACATTTGGTTCAGAAGCCACGATAACCGGCGTTGAACCAAGGCTAAAAGCCATAAT
CTCAGTGTTGGCTCGGCTCCAAGCCATGGCTGCTAACGTCCGATGAGCCAAGCCTCGGCT
GAGAGTGAAAAGACTGCCGAATACTGGTATGCCACGAGGACCAGGGATCGCGACACGACC
CAACCGGTTACGGCCGTTCTTCCAAGCGACACCACCGCCTCCGGCGAGAGCCCACGTGAC
TATAGAGAGAAAAACGACGGCAAGGAATAGATAGCCTAGGGTGGAAACATCATGTAGGTT
TTGGGATCCAAAACCGGCAGGCAGTGCAATCATCCAATAGCTTGTTTCTTTTGAAGCCAA
ATTCATCAACTCCATTATTTTGGGTTGATGTACTTTTTTTTTTTGTAATCTTGTTTCTGG
TCAAATAATGAACAACGTACGAACCAATAACAATAACAAAAGGTTTTTCAAGAGAAAGCA
AAAAAATCCTATGAAAAGGAAAGTACTCTTTTTTTTTGGTTGAAAAAAAAATTACTTGGA
AAAAAACATGGAAAAAT

Last Updated on 06.12.2018 16:11