Sequences from confirmation amplicon


Confirmation sequences for line 632G08 and hit At2g35640 are shown.
Line ID 632G08
Locus At2g35640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14982872
Primers used for PCR 27sf - 8474, show primer details
   
Sequence Name 36-K116518-0022-632-G08C-27sf
GenBank Accession LN502366 [GenBank]
Sequencing primer gene specific primer, 27sf: 5'-CCTTCCCACTAAATATAACGACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116518-0022-632-G08C-27sf
CCTTCTGTGATAATCTCGGGGTTATACGTTGCTAAAAGTTGACTCCTAGTCACCAAATGG
CATAGTTTTATAATAACACTGTTAGATCCTTGTACCAGTTACTCAGTGAGATCCTTAGAT
TCACATAGAGTTGTCCCATATAAAGTAAAAAAAACTAGAGACATGAAACATAGATTTTTT
TTTTTCATCACGTGAAACATAGATAAGAAAAGAAAAAGGATAGGTGTTCAATTATTTAAT
TTAGTAGGAAACATAGAATAAAAAAACTTGCTTTGTTTTTCATATTTTCTCATTATGGGA
ATCACTGTCTTTTCAAACTTGGTGACAATCTTAGTACTAGAAAACAAAAGACTTTGACCG
GTCTTTGGCTCATCTCTTTCTTCTCCCCTTTCTCCAACACTTATTTCTTTCTCTCTCTAG
TTCAGGAATTCAAGATTCAAAGACACACGTATGGCTACCTCTGGGTAAATCCAAAGAGAC
ACATATTTGGCTGACGCTAATCCATCGAGTGGTGAACAGATGGTGTACACATATATTTGG
TCAGGTTGTTTTCTGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACTTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAAATTTCCG
   
Sequence Name 10-K116517-0022-632-G08C-8409
GenBank Accession LN490821 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K116517-0022-632-G08C-8409
TCATTGAATATATCCTGTACAGAAAACAACCTGACAAGATATATGTGTACACTATCTGTT
CACCACTCGATGGATCAGCGTCAGCCATATATGTGTCTCTTTGGATTTACCAAGAGGTAG
CCATACGTGTGTCTTTGAATCTTGAATTCATGAACTAGAGAGAGAAAGAAATAAGTGTTG
GAGAAAGGGGAGAAGAAAGAGATGAGCCAAAGACCGGTCAAAGTCTTTTGTTTTCTAGTA
CTAAGATTGTCACCAAGTTCGAAAAGACAGTGATTCCAATAATGAGAAAATATGAAAAAC
AAAGCAAGTTTTTTTATTCTATGTTTCCTACTAAATTAAATAATTGAACACCTATCCTTT
TTCTTTTCTTATCTATGTTTCACGTGATGAAAAAAAAAAATCTATGTTTCAGGTCTCTAG
TTTTTTTTACTTTATATGGGACAACTCTATGTGAATCTAAGGATCTCACTGAGTAACTGG
TACAAGGATCTAACAGTGTTATTATAAAACTATGCCATTTGGTGACTAGGAGTCAACTTT
TAGCAACGTATAACCCCGAGATTATCACAGAAGGCCATGCTCAGCAGTGGTCTTAGACTC
TTAGTACTTGACATGGTCGTTATATT

Last Updated on 06.12.2018 16:11