Sequences from confirmation amplicon


Confirmation sequences for line 633A08 and hit At1g14590 are shown.
Line ID 633A08
Locus At1g14590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5000196
Primers used for PCR 51sm - 8474, show primer details
   
Sequence Name 69-K116549-0022-633-A08C-51sm
GenBank Accession LN512272 [GenBank]
Sequencing primer gene specific primer, 51sm: 5'-CAGCGTTTTGTAGTGGGTGTATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K116549-0022-633-A08C-51sm
GGCAAAATCCTAACGTTGATCGTAAGTGACGATGGTCGGTGGTGATCGAATCTATCTCAC
CGTGTCGGTTCGAGATTCCACACGGCGAGCCTCGAGGCTTCGTCTTCAGCTAATAATCCC
TTCGCCGAGGGTTCCGGCGAGATGTCTCCGGGACCATCAATCCCGCTCCGCCGAGCTGCT
CTATTTCTAGCTGCCATCTCCATCTCCTGCTTCGTCCTTTACAGAGCCGCTGATTCCTTG
TCTTTCTCCCCTCCCATTTTCGATCTCTCTTCTTATCTGGTTAGTCTTCTCTTCTCTTCA
CCACAAGTTCTTCGCGAATTTGATGCTCGTCGTTATTAAACGCACGATTTAGAGATTTTA
GGTTCGATTGAAATTGCTGTGAAATTTGAAATAACATAGCTGGTTCTGGTGCTAAGAGAT
TGCATGTGGATTGTTCTTATGAACTAGCTCAGTGTTACTGCGATTTATGTTCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGC
   
Sequence Name 29-K116548-0022-633-A08C-8409
GenBank Accession LN499446 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116548-0022-633-A08C-8409
CCTTTACATTGAATATATCCTGAACTAAATCGCAGTAACACTTAGCTAGTTCATAAGAAC
AATCCACATGCAATCTCTAAGCACCAGAACTAGCTATGTTATTTCAAATTTCACAGCAAT
TTCAATCGAACCTAAAATCTCTAAATCGTGCGTTTAATAACGACGAGCATCAAATTCGCG
AAGAACTTGTGGTGAAGAGAAGAGAAGACTAACCAGATAAGAAGAGAGATCGAAAATGGG
AGGGGAGAAAGACAAGGAATCAGCGGCTCTGTAAAGGACGAAGCAGGAGATGGAGATGGC
AGCTAGAAATAGAGCAGCTCGGCGGAGCGGGATTGATGGTCCCGGAGACATCTCGCCGGA
ACCCTCGGCGAAGGGATTATTAGCTGAAGACGAAGCCTCGAGGCTCGCCGTGTGGAATCT
CGAACCGACACGGTGAGATAGATTCATCACCACCGACCATCGTCACTTACGATCAACGTT
AGGATTTTGCCCAATAATTTTAATTTCTATTATTAATCATCAATTTTTGCAAACATAAAC
GGTTACAAATTTAATGTGTTCCATAAATTATACACCC

Last Updated on 06.12.2018 16:11