Sequences from confirmation amplicon


Confirmation sequences for line 633G06 and hit At5g59040 are shown.
Line ID 633G06
Locus At5g59040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23837167
Primers used for PCR 26jn - 8474, show primer details
   
Sequence Name 47-K113214-0022-633-G06C-8409
GenBank Accession LN506250 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K113214-0022-633-G06C-8409
TTCATCACATTGTTGATTTTTGTCTTAGATCGTTTTTATTTTCCTTGTTTCCCGTTGAAA
TAATGATTAGGAAAATAAAAAAATTCACGTTTTTGGATTTTAGATTGAGTAAGAATGTAA
GATTGGAAGTTCTTTAACCTCATTTTATTTTATTTTGTTTTGATCTCTAATATATAATTG
TGTTGGTCAAATTTCTAAGAATTGTATAATAGAATTTTTTTTTTTTAAATATACAACTTT
CCTTCAAACGGTAGTATATACGGTATATTTAAAAATCATACCTAAAAAAAATTTGTTCTT
TTGAAACTAGGGGTTAAATATTTTTGTATTGCTAACAAAAGATTTGAACAGGGTAATGAC
TTCGTAAATGAAAAAATGTATAATTTCCTATCCCCCATCTCGAAAAATTTTGTTATGAAA
TTCCCAAAAATTTTCAAACTTTTCAAAAATCAGGGCTTCCTAAAACAATAACAACCAATC
TAAATGTTCGTTCCAATTTAAATTCT
   
Sequence Name 47-K113215-0022-633-G06C-26jn
GenBank Accession LN506251 [GenBank]
Sequencing primer gene specific primer, 26jn: 5'-AGAGAATTTAGATCGGAACGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K113215-0022-633-G06C-26jn
TTAGAGCCTGATTATGAACGTTTGAAGTTCTTGGGAATTTCATAACAAACTATTACGAGA
TGGGCGATAGGAAGTTATACATTTTTTCATTTACGATGTCATTACCTTGTTCAAATCTCT
TGTTAGCAATACAAATATATTCAACCACTAGTTTCAAAAGAACAAATTTCTCTAAGCTAT
GATTTTTAACTATACCGTATATACTACCGTTCGAAGGAAAGTTGTATATTCAAAAAAAAA
AAATTCTATTATACAATTCTAAAAATTTTGACCAACACATTTATTTTTTAAAGATCAAAA
CAAAATAAAATAAAAGGAGGTTAAAAAATTTCCAATCTTACATTCTTACTCAATCTAAAA
TCCAAAAACGGGAATTTTTTTATTTTCCAAATCATTATTTCAACGGGAAACAGGGAAAAT
AAAAACGATTTAAAACAAAAATCAACAATGTGATTGAACCTCGGGGTGACTGTTACAAAA
TCATAAAGCATTGAGTATGAGGGTCAATATGGAAAAAAAAAAAGAGTATTTCCCATTTTT
TTTTCATTTCAAAAATGTAAATTTCC

Last Updated on 06.12.2018 16:11