Sequences from confirmation amplicon


Confirmation sequences for line 634A06 and hit At4g15396 are shown.
Line ID 634A06
Locus At4g15396 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8807940
Primers used for PCR CH89 - 8474, show primer details
   
Sequence Name 14-K107031-0022-634-A06C-8409
GenBank Accession LN492530 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K107031-0022-634-A06C-8409
GCCATTTACATTGAATATATCATTGCTTCATTACAATTTAATTATAAAAAAAATTAAAAA
TCTAAACACATATCTATTAAATAAAGAGAAATAACAAAACTGTGGATGTTCTTACTTCCT
TATAATAAATGAGCGGATGTTTTTGTATATAGTTGTATCCTACATGTAAAACAAATTGGA
TAATTTTTAAAGAAATGATTAACTGTATCTCGTAAATATTTTACTAGATGGAATTTATAT
ACATAATCAAATTTCAAAGAACAAAGAAAAATCGTAATAAATTAAACTTCTCTTTGGATA
ACATTTCAGCGAAAAAAGAAGAAGCAAGTTAAGGTTTGCTTCTTGAGAATATATTGTTGA
AGTGTTTTTTATTTGAAAATTCCCACAATTTATTAAGAAAAAAATTAACGATTTGCACCC
GAACATATCTAGTCCTCATAATCAATTCCTCCGAGTTTTATTAGTAGATTACCGTGTCTC
AATTTTTGCATTTTTTTCCCCCAAAAAAAATAGACATGGACCAGTTTTTAGGACAAGCTT
ATTTGGAGGAAAAGTTATAATTTCGACTGATATGGGGTTGAA
   
Sequence Name 30-K107032-0022-634-A06C-CH89
GenBank Accession LN499741 [GenBank]
Sequencing primer gene specific primer, CH89: 5'-GTTCAACCCAATATCAGTCGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K107032-0022-634-A06C-CH89
ATAGCTTGTCCTAAAACTGGTCCATGTCTATTTTTTTTGGGGGAAAAAAATGCAAAAATT
GAGACACGGTAATCTACTAATAAAACTCGGAGGAATTGATTATGAGGACTAGATATGTTC
GGGTGCAAATCGTTAATTTTTTTCTTAATAAATTGTGGGAATTTTCAAATAAAAAACACT
TCAACAATATATTCTCAAGAAGCAAACCTTAACTTGCTTCTTCTTTTTTCGCTGAAATGT
TATCCAAAGAGAAGTTTAATTTATTACGATTTTTCTTTGTTCTTTGAAATTTGATTATGT
ATATAAATTCCATCTAGTAAAATATTTACGAGATACAGTTAATCATTTCTTTAAAAATTA
TCCAATTTGTTTTACATGTAGGATACAACTATATACAAAAACATCCGCTCATTTATTATA
AGGAAGTAAGAACATCCACAGTTTTGTTATTTCTCTTTATTTAATAGATATGTGTTTAGA
TTTTTAATTTTTTTTATAATTAAATTGTAATGAAGCAATGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAATAAAAGAGCGGGTGTTTTTGTATATAGTTGTATCCTACATGTAAAACAAATTGGATA
ATTTTTAAAGAAATGATTAACTGTATCTCGTAAATATTTTACTAGATGGAATTTATATAC
ATAATCAAATTTCAAAGAACAAAGAAAAATCGTAATAAATTAAACTTCTCTTTGGATAAC
ATTTCAGCGAAAAAAGAAGAAGCAAGTNAAGGTTTGCTTCTTGAGAATATATTGTTG

Last Updated on 06.12.2018 16:11