Sequences from confirmation amplicon


Confirmation sequences for line 634C06 and hit At1g45050 are shown.
Line ID 634C06
Locus At1g45050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17032365
Primers used for PCR AB08 - 8474, show primer details
   
Sequence Name 29-K024451-022-634-C06C-8409
GenBank Accession LN499127 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K024451-022-634-C06C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTAATTGGCAATATA
TCATGTAGATAAGCCATTTCATATCTTAAACAATGAAGTATCATAGATACTAAGGAAAAG
GATCTACACGCATGGGGGTATACAATAAAAACAGTTTTGAGTGATAACACTTATCAATAA
TCATCAACATATCTTAAAACTCAGCAATATTCACTTTCTTGTTTGACCAGAAAACTATGA
AATGCAGAGTCTAACCGATTCTACATCTACATATCAATTCGACAAACCCTTGCTTTGATC
AAAACTAAACATAAGCATATGCAGAGCAGACAGTAACCAAGAGATAGCAAGCAATACCTA
AACAAATATGTCCATTGCTGTATATATGTGGATGTGAAGGTGCCGGAGAAACAAACACTA
CCTGCTCAACACAACAGGGAAAAATAAGTCATTCTCCTCCACAGTAGCTCCAATCCAAGA
GACACCAAAACATAAAACAAAAAACTTTTTTACAATAAGTTCGAATTAAAACATGAAACA
TCCATAACAAAGTCTCTCTACAGAAGCAAACCCAAGAATTTTATCAGTGAGAGACTTCT
   
Sequence Name 30-K024451-022-634-C06C-AB08
GenBank Accession LN499563 [GenBank]
Sequencing primer gene specific primer, AB08: 5'-TCTTTGGTTGGCTAGAAGTCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K024451-022-634-C06C-AB08
CTGATAAAATTCTTGGGTTTGCTTCTGTAGAGAGACTTTGTTATGGATGTTTCATGTTTT
AATTCGAACTTATTGTAAAAAAGTTTTTTGTTTTATGTTTTGGTGTCTCTTGGATTGGAG
CTACTGTGGAGGAGAATGACTTATTTTTCCCTGTTGTGTTGAGCAGGTAGTGTTTGTTTC
TCCGGCACCTTCACATCCACATATATACAGCAATGGACATATTTGTTTAGGTATTGCTTG
CTATCTCTTGGTTACTGTCTGCTCTGCATATGCTTATGTTTAGTTTTGATCAAAGCAAGG
GTTTGTCGAATTGATATGTAGATGTAGAATCGGTTAGACTCTGCATTTCATAGTTTTCTG
GTCAAACAAGAAAGTGAATATTGCTGAGTTTTAAGATATGTTGATGATTATTGATAAGTG
TTATCACTCAAAACTGTTTTTATTTGTATACCCCCATGCGTGTAGATCCTTTTCCTTAGT
ATCTATGATACTTCATTGTTTAAGATATGAAATGGCTTATCTACATGATATATTGCCAAT
TAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGN
AGTATAAATGGTCAATATNGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11