Sequences from confirmation amplicon


Confirmation sequences for line 634F06 and hit At2g04050 are shown.
Line ID 634F06
Locus At2g04050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1339011
Primers used for PCR KS55 - 8474, show primer details
   
Sequence Name 54-K101502-0022-634-F06C-8409
GenBank Accession LN508424 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K101502-0022-634-F06C-8409
GCCATTTACAATTGAATATATCCTGATAACTACTCTCTCCTCTTAAGTTCAAGATCAAGT
TGCCTCTCAAATCTTGATAACACTATATTAATTCTTTGTGCAGTTTGGGTTAGCGGGTGC
ACTAGAAACTCTTTGTGGCCAAGCTTATGGAGCCAAACAATACGAAAAAATCGGAACCTA
CACATACTCTGCAACTGCCTCTAACATCCCTATATGTGTCCTCATATCAGTTCTCTGGAT
TTACATCGAAAAGCTCTTAATCTCTCTCGGTCAAGACCCTGACATCTCTAGAGTCGCTGG
CTCCTATGCCTTGTGGCTCATACCGGCTTTGTTCGCCCATGCGTTTTTCATCCCACTGAC
TCGGTTTCTGCTAGCACAAGGGTTGGTTCTTCCACTGCTCTACTGTACCCTGACCACTCT
NTTGTTCCACATCCCTGTTTGCTGGGCTTTTGTTTACGCGTTTGGTCTGGGAAGCAATGG
AGCTGCCATGGCTATTAGTGTGTCTTTCTGGTTCTATGTTGTGATTCTCTCANGTTATGT
G
   
Sequence Name 54-K101506-0022-634-F06C-KS55
GenBank Accession LN508425 [GenBank]
Sequencing primer gene specific primer, KS55: 5'-GCTGGAGTACCTCACATAACATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K101506-0022-634-F06C-KS55
CTAATAGCCATGGCAGCTCCATTGCTTCCCAGACCAAACGCGTAAACAAAAGCCCAGCAA
ACAGGGATGTGGAACAAAAGAGTGGTCAGGGTACAGTAGAGCAGTGGAAGAACCAACCCT
TGTGCTAGCAGAAACCGAGTCAGTGGGATGAAAAACGCATGGGCGAACAAAGCCGGTATG
AGCCACAAGGCATAGGAGCCAGCGACTCTNGAGATGTCAGGGTCTTGACCGAGAGAGATT
AAGAGCTTTTCGATGTAAATCCAGAGAACTGATATGAGGACACATATNGGGATGTTAGAG
GCAGTTGCAGAGTATGTGTAGGTTCCGATTTTTTCGTATTGTTTGGCTCCATAAGCTTGG
CCACAAAGAGTTTCTAGTGCACCCGCTAACCCAAACTGCACAAAGAATTAATATAGTGTT
ATCAAGATTTGAGAGGCAACTTGATCTTGAACTTAAGAGGAGAGAGTAGTTA

Last Updated on 06.12.2018 16:11