Sequences from confirmation amplicon


Confirmation sequences for line 634G12 and hit At3g45410 are shown.
Line ID 634G12
Locus At3g45410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16655233
Primers used for PCR EU25 - 8474, show primer details
   
Sequence Name 52-K103393-0022-634-G12C-EU25
GenBank Accession LN507784 [GenBank]
Sequencing primer gene specific primer, EU25: 5'-TCCATTGATCTCTCTCATGCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103393-0022-634-G12C-EU25
GGGTTCGCACTAACAAATGGGACTTCCTCTTCACATCTGCTAGCTATTGAGCTCGATACC
GTTAAGACAGTAGAGTTTAATGAGCTGGAGAAGCCTCATGTTGGTATTGATTTGAACAGC
CCAATATCTGTTGAATCTGCTCTGCCATCTTACTTTTCCAACGCTTTGGGGAAGAATATA
AGCATAAATCTTCTTAGTGGGGAGCCTATACNGGTCTGGGTAGATTACGATGGCTCGTTT
CTAAATGTTACACTGGCCCCTATNGAAATCAAGAAGCCAAATCAGCCTCTTATATCANGA
GCCATCAATCTTTCAGAAATCTTTCAAGAGAAGATGTATGTTGGGTTCTCTTCATCAACA
GGGAATCTTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCANAAATGTAG
   
Sequence Name 42-K103394-0022-634-G12C-8409
GenBank Accession LN504430 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K103394-0022-634-G12C-8409
GAGCCTTTACAATTGAATATATCCTGAGAAGATTCCCTGTTGATGAAGAGAACCCAACAT
ACATCTTCTCTTGAAAGATTTCTGAAAGATTGATGGCTCTTGATATAAGAGGCTGATTTG
GCTTCTTGATTTCTATAGGGGCCAGTGTAACATTTAGAAACGAGCCATCGTAATCTACCC
AGACCTGTATAGGCTCCCCACTAAGAAGATTTATGCTTATATTCTTCCCCAAAGCGTTGG
AAAAGTAAGATGGCAGAGCAGATTCAACAGATATTGGGCTGTTCAAATCAATACCAACAT
GAGGCTTCTCCAGCTCATTAAACTCTACTGTCTTAACGGTATCGAGCTCAATAGCTAGCA
AATGTGAAGAGGAAGTCCCATTTGTTAAGTTGCTGAAGACCCCCNAGTACTGAGTTGCGT
ATGCCTGAGAGAGATCAATGG
Primers used for PCR TH45 - 8474, show primer details
   
Sequence Name 70-K118414-0022-634-G12C-TH45
GenBank Accession LN512465 [GenBank]
Sequencing primer gene specific primer, TH45: 5'-GAACCAAGTTCCGATGTTGTAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118414-0022-634-G12C-TH45
TTCTCCCTTGCTCGCATCGTGGGATAGTCTTTTCACAGCTATATGCCTGCCTCCGGGCAA
AGTTCCTTTGTAGACTTCTCCAAACCCACCTTTCCCAACACGACAATCTTTACGAAACCC
ATTTGTTGCTTTGTACAGAGACTTATAGGAGAATCGGTGTGGGCCGTATTCCTTTTCCCA
CCATTCTTTTACTTCTGCATATTTCTTTCTCCTGTACCAATAAACTCCTCCAAGAACCAT
CACCACAGGAATCACTAATAAGATGACCAAACCAATAAGTAATGGAGATAATTTCTTTTT
TTCCTCTTTAGGAAGAGGAACCCGAGGAAGTGTAGAGAGGTTCAGGCTCTGTAATTGTTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGGTTATTAT
   
Sequence Name 22-K118413-0022-634-G12C-8409
GenBank Accession LN496314 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118413-0022-634-G12C-8409
ATTGAACAATTACAGAGCCTGAACCTCTCTACACTTCCTCGGGTTCCTCTTCCTAAAGAG
GAAAAAAAGAAATTATCTCCATTACTTATTGGTTTGGTCATCTTATTAGTGATTCCTGTG
GTGATGGTTCTTGGAGGAGTTTATTGGTACAGGAGAAAGAAATATGCAGAAGTAAAAGAA
TGGTGGGAAAAGGAATACGGCCCACACCGATTCTCCTATAAGTCTCTGTACAAAGCAACA
AATGGGTTTCGTAAAGATTGTCGTGTTGGGAAAGGTGGGTTTGGAGAAGTCTACAAAGGA
ACTTTGCCCGGAGGCAGGCATATAGCTGTGAAAAGACTATCCCACGATGCAGAGCAAGGG
ATGAAACAGTTTGTGGCAGAAGTTGTAACCATGGGAAATTTACAACATCGGACCCTTGGT
T

Last Updated on 06.12.2018 16:11