Sequences from confirmation amplicon


Confirmation sequences for line 635H12 and hit At3g56520 are shown.
Line ID 635H12
Locus At3g56520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20947229
Primers used for PCR 21lu - 8474, show primer details
   
Sequence Name 43-K114144-0022-635-H12C-21lu
GenBank Accession LN504915 [GenBank]
Sequencing primer gene specific primer, 21lu: 5'-CTTTAATCATCCTGTGAAAGAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114144-0022-635-H12C-21lu
CCAGTGAGACATTGGTACTATTTTTGCAGAAAACGTGACAACCAAGTTACTAGTAACTCT
CATAATCTCTGGACACCAATCGGAGAAGAAACCAATGTGCTAGATCCAAAGAATAATGAT
GCATTAGTTGGTATTAAGCGTCGGTTTACTCTTATTGCGCAAGAAGAAGAAGAATCTGAT
AATATTTGTTTATCTGATGATGAAGAAGCTTTAAAGTATAATTGGTTCAGCAATGGATCA
GCAATGAGTATGACCATATTTAATTGTAAATGGCTTCATGTCCGGGATATAGCCATTTAA
ATTGTAAATGGCTTCATGTCCGGGATATTTATCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 16-K114143-0022-635-H12C-8409
GenBank Accession LN493516 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114143-0022-635-H12C-8409
CATTTCATTGAATATATCCTGATAATATCCCGGACATGAAGCCATTTACAATTTAAATGG
CTATATCCCGGACATGAAGCCATTTACAATTAAATATGGTCATACTCATTGCTGATCCAT
TGCTGAACCAATTATACTTTAAAGCTTCTTCATCATCAGATAAACAAATATTATCAGATT
CTTCTTCTTCTTGCGCAATAAGAGTAAACCGACGCTTAATACCAACTAATGCATCATTAT
TCTTTGGATCTAGCACATTGGTTTCTTCTCCGATTGGTGTCCAGAGATTATGAGAGTTAC
TAGTAACTTGGTTGTCACGTTTTCTGCAAAAATAGTACCAATGTTCTTCACTTGGATAAC
CTGAAAGAAGGAAACAAAGAAAATTCAGTTGGCTCTTTCACAGGAGGATTAAA

Last Updated on 06.12.2018 16:11