Sequences from confirmation amplicon


Confirmation sequences for line 637E03 and hit At1g54090 are shown.
Line ID 637E03
Locus At1g54090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20189757
Primers used for PCR DN38 - 8474, show primer details
   
Sequence Name 49-K115714-0022-637-E03C-DN38
GenBank Accession LN506944 [GenBank]
Sequencing primer gene specific primer, DN38: 5'-TCTCGTATACTGCTTGTGGATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K115714-0022-637-E03C-DN38
GGAATCTGAACCGGAACCATCTGGTAAACCAGATCCACTTGTTGTTGCTCTTCTTCTTCG
TTACCGTCATCGCAAGTGTCATCTTCAAGCTCCGTACGAGAAGAAGAAGCGAAAGAGGAA
GAAGATAACGATGAATCGAGAAGAAGAGAATCAGGTTCGAAAGTAGAAGTCTGAGAGATT
AAGATATTTCTTAGCTCGTCCTCGAGTCTAGCCATGGCGATTTGAATCGTTGCTTTGACT
TCATCGGAGATTGAGATTGAAGAGATGTGTCTTTGAACTTCATCTACAGCTTTCAAGTAC
CGATCTACTTCTTCTCGATCACCACCGCTTTGGAAAATCAGATTCTCTTTAGCTTCTTCA
GATGTTGTTGAATCCCACCGGAGTATTATCTTCTCCGCTGTCTCTAAATCGGAATCAACG
CCNGTTCCAGTTTCAGATTTCACGATCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 09-K115713-0022-637-E03C-8409
GenBank Accession LN490329 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115713-0022-637-E03C-8409
ATTTCATTGAATATATCCTGATCGTGAAATCTGAAACTGGAACTGGCGTTGATTCCGATT
TAGAGACAGCGGAGAAGATAATACTCCGGTGGGATTCAACAACATCTGAAGAAGCTAAAG
AGAATCTGATTTTCCAAAGCGGTGGTGATCGAGAAGAAGTAGATCGGTACTTGAAAGCTG
TAGATGAAGTTCAAAGACACATCTCTTCAATCTCAATCTCCGATGAAGTCAAAGCAACGA
TTCAAATCGCCATGGCTAGACTCGAGGACGAGCTAAGAAATATCTTAATCTCTCAGACTT
CTACTTTCGAACCTGATTCTCTTCTTCTCGATTCATCGTTATCTTCTTCCTCTTTCGCTT
CTTCTTCTCGTACGGAGCTTGAAGATGACACTTGCGATGACGGTNACGAAGAAGAAGAGC
AACAACAAGTGGATCTGGTTTTACCAGATGGTTCCGGTTCAGATTCCGGTTCGAGACGGT
TATCGAGGAGTAGGAGAAGCAATAGCAAATCCACAAGCATAATACGAGA

Last Updated on 06.12.2018 16:11