Sequences from confirmation amplicon


Confirmation sequences for line 637G05 and hit At2g44440 are shown.
Line ID 637G05
Locus At2g44440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18337917
Primers used for PCR HP43 - 8474, show primer details
   
Sequence Name 62-K033718-022-637-G05C-8409
GenBank Accession LN510681 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K033718-022-637-G05C-8409
TTACAATTGAATAATCCTGACATAGGAACAGGTCCTCGTTTCGTTGTCGATGAAGCAAAC
GGGTGAGTGGGATCAGCTTGACGATAAAAAGAAGGTGAAGAACTAGCGAATGGTTGAGAA
GGAATTGGCTGGTTTGGTTTGTGCGTCTTCATAGAAGCTGAAACAGAAGGGCTTGGCAAT
GTATCGTGAACCACTTGAGCAGCATTGCGCATACTTGGCTGCATTCCTCCAGATTGTCTC
CATTCCCTAATAACCGAAAAAGCATTTGTTATTGATAGGAAAAGAGAAGAAGCTTTTCAC
TTGCTCATCGGATAAATGCTCAGATTTAAGATCTTGCCTTATCCTTCGTATTGTATCATC
TGAGTTAACACGG
   
Sequence Name 24-K033718-022-637-G05C-HP43
GenBank Accession LN496901 [GenBank]
Sequencing primer gene specific primer, HP43: 5'-GCCGTGTTAACTCAGATGATACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K033718-022-637-G05C-HP43
TTAATCTGAGCATTTATCCGATGAGCAAGTGAAAAGCTTCTTCTCTTTTCCCCCTATAAC
AAATGCTTTTTCGGTTATTAGGGAATGGAGACCCTCAGGAGGAATGCAGCCAAGTATGCG
CAATGCTGCTCAAGTGGTTCACGATACATTGCCAAGCCCTTCTGTTTCAGCTTCTATGAA
GACGCACAAACCAAACCAGCCAATTCCTTCTCAACCATTCGCTAGTTCTTCACCTTCTTT
TCATCCTCAAGCTGATCCCACTCACCCGTTTGCTTCATCGACAGCTAAACGAGGACCTGT
TCCTATTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTCACGTCCAAAATGG

Last Updated on 06.12.2018 16:11