Sequences from confirmation amplicon


Confirmation sequences for line 639C04 and hit At1g73870 are shown.
Line ID 639C04
Locus At1g73870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27780290
Primers used for PCR BA24 - 8474, show primer details
   
Sequence Name 50-K105015-0022-639-C04C-BA24
GenBank Accession LN507162 [GenBank]
Sequencing primer gene specific primer, BA24: 5'-TTGAAGAGGGTGAAAGATGAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K105015-0022-639-C04C-BA24
ATCAAGGATAGTGATAGAGAAGCATCAAATGATAAAGATAGAAAGACAAGTTTGTTTCTG
AGATTAGATTATGGAGCTGTGATTAGTGCTTGGGATAATCACGGTTCGCCGTGGAAAACC
GGAATCAAACCGGAGTGCATGGTAAGTTGGTGTCTCTAGCTATATCTCTCATCTCTATGT
TAGTCTTTAGTAGTGTCGAGTTCCGTTGACTTTGAGCTGATATATATTGATTATAAAAGA
CGAAATAACGTTATAAAGTTCACTGATCCAGTTGGGAGGGAACACGTGTCTGCCTCATGT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 34-K105016-0022-639-C04C-8409
GenBank Accession LN501409 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K105016-0022-639-C04C-8409
ATTTCACATGAGGCAGACCGTGTTCCCTCCCAACTGGATCAGTGAACTTTATAACGTTAT
TTCGTCTTTTATAATCAATATATATCAGCTCAAAGTCAACGGAACTCGACACTACTAAAG
ACTAACATAGAGATGAGAGATATAGCTAGAGACACCAACTTACCATGCACTCCGGTTTGA
TTCCGGTTTTCCACGGCGAACCGTGATTATCCCAAGCACTAATCACAGCTCCATAATCTA
ATCTCAGAAACAAACTTGTCTTTCTATCTTTATCATTTGATGCTTCTCTATCACTATCCT
TTGATCCTCCATTTTCACACTTGGCTTCTTCTTCTTCTTCATCTTCATCTTTCCCCCTCT
TCA

Last Updated on 06.12.2018 16:11