Sequences from confirmation amplicon


Confirmation sequences for line 640A10 and hit At4g39740 are shown.
Line ID 640A10
Locus At4g39740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18436486
Primers used for PCR 11ax - 3144, show primer details
   
Sequence Name 09-K106867-0022-640-A10C-3144
GenBank Accession LN490198 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106867-0022-640-A10C-3144
AGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGCCCATGACAGGGNACCCGGGGAN
CAGAANGNCGNTTCCCGCCTTCGGTTTAACTATCAGTGTTTGAATTGAATCATGTAGATT
GTAGATTGACGGCGGATTGTTTATTGCTTGACTTGCTTGCTCTCCTTTCGTCGTTGTAAT
GAAGAAAGCCGACGAATCCGGCAAAACCAAGTAGAATCGCAGGCTGAAAATGCAGACAAA
AAAGGGAACATAAGTCCCAAATGAACGATCCAATTCAAGAACACAAGTGAAGGCAACAAG
AGTTTTTAGGATCTATACAACAGCATAACGAGCACGAGAACGAGAAGGAGCTTGAGTTCC
TCCTCCTGTGGGAAAAAGAGGTTTCACATCTGGAATTTCATGGCCCTGTCTTGTTGATTT
ACAGTAATTGACTGATTGAATTCGTTTCGATGGTCCACACCTAAAAAAAAAATCAACGCT
AAGATTCAATATAATGTTTTAAGATCAAGAATTTCGATACTTTAGAAGATAAACCAAACA
AGAAACATTCACAAGACCATCAAAATTCCAACAAAGAGAGACTTTTTTCGCAGATAGAGT
AACAATTGTTCTACTTAAAAAACCCAGATAAAGTAAAATCCCCTAAGCAACTTAAAATTA
GGGACGGGCCCTCCTATTA
   
Sequence Name 19-K106868-0022-640-A10C-11ax
GenBank Accession LN494778 [GenBank]
Sequencing primer gene specific primer, 11ax: 5'-CCCTAATTTTAAGTTGCTTAGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K106868-0022-640-A10C-11ax
CATTGTACTCTATCTGCGAAAAAAGTCTCTCTTTGTTGGAATTTTGATGGTCTTGTGAAT
GTTTCTTGTTTGGTTTATCTTCTAAAGTATCGAAATTCTTGATCTTAAAACATTATATTG
AATCTTAGCGTTGATTTTTTTTTTAGGTGTGGACCATCGAAACGAATTCAATCAGTCAAT
TACTGTAAATCAACAAGACAGGGCCATGAAATTCCAGATGTGAAACCTCTTTTTCCCACA
GGAGGAGGAACTCAAGCTCCTTCTCGTTCTCGTGCTCGTTATGCTGTTGTATAGATCCTA
AAAACTCTTGTTGCCTTCACTTGTGTTCTTGAATTGGATCGTTCATTTGGGACTTATGTT
CCCTTTTTTGTCTGCATTTTCAGCCTGCGATTCTACTTGGTTTTGCCGGATTCGTCGGCT
TTCTTCATTACAACGACGAAAGGAGAGCAAGCAAGTCAAGCAATAAACAATCCGCCGTCA
ATCTACAATCTACATGATTCAATTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGA
CAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGA
AGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACA
TCAATCC
Primers used for PCR AU22 - 8474, show primer details
   
Sequence Name 29-K106287-0022-640-A10C-AU22
GenBank Accession LN499300 [GenBank]
Sequencing primer gene specific primer, AU22: 5'-GAAATGGCTGGAGCTTTGTATAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K106287-0022-640-A10C-AU22
CTTTGGACTATCTTTAGTTGCTCAGGTCCACATCCGGGGAAAAAGAGTATCCAAAGTAGA
GTAGAACCCATTTACCACAAAAGTCATTCTCTGTGACTATCTTGTTTTCTGTGCTCACAA
GCGTAAACGGACCTCCGATAATCGGACCTTTGACAGTTGTATTAGAACCGCAGCCACAAC
CGCTATTGCTGCTTGCTTGACCTGCAAAAGTAGAAGCATGAAACATCTCAGCTTCATCAC
TCCAATGTGTCAAAAAACCATCATCAAAGTCTATAGTACCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 29-K106286-0022-640-A10C-8409
GenBank Accession LN499299 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K106286-0022-640-A10C-8409
GGAATGAGCCTTTACAATTGAATATATCCTGGTACTATAGACTTTGATGATGGTTTTTTG
ACACATTGGAGTGATGAAGCTGAGATGTTTCATGCTTCTACTTTTGCAGGTCAAGCAAGC
AGCAATAGCGGTTGTGGCTGCGGTTCTAATACAACTGTCAAAGGTCCGATTATCGGAGGT
CCGTTTACGCTTGTGAGCACAGAAAACAAGATAGTCACAGAGAATGACTTTTGTGGTAAA
TGGGTTCTACTCTACTTTGGATACTCTTTTTCCCCGGATGTTGGACCTGAGCAACTAAAG
ATGATGTCCAAAGCTGTTGATAAACTAGGTTGAGTTACTTATACAAAGCTCCAGCCATTT

Last Updated on 06.12.2018 16:11