Genotyping and primer details


Genotyping and primer details for 640A10 - At4g39740
Line ID 640A10
Locus At4g39740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18436486 of line 640A10 Chr4:18436486
   
Insertion allele amplicon 3144 - 11ax
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 11ax: 5'-CCCTAATTTTAAGTTGCTTAGGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18437001
   
Insertion allele amplicon 8474 - AU22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AU22: 5'-GAAATGGCTGGAGCTTTGTATAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18436156
   
wt amplicon (over InsPos) 11ax - AU22
wt amplicon start Chr4:18436156
wt amplicon end Chr4:18437001
wt amplicon size 846 bp
Locus specific primer AU22: 5'-GAAATGGCTGGAGCTTTGTATAAG-3'
2nd locus specific primer 11ax: 5'-CCCTAATTTTAAGTTGCTTAGGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11