Sequences from confirmation amplicon


Confirmation sequences for line 640B04 and hit At3g63440 are shown.
Line ID 640B04
Locus At3g63440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23425635
Primers used for PCR TH19 - 8474, show primer details
   
Sequence Name 42-K103676-0022-640-B04C-TH19
GenBank Accession LN504435 [GenBank]
Sequencing primer gene specific primer, TH19: 5'-GGCGAGATCCTAAACTGTACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K103676-0022-640-B04C-TH19
ATGGTGTTCTTGGTGGTTAGGTCAGTTTGGCATCATAACGCGGGCAAGAATAGCATTGGA
ACCAGCACCAACCATGGTAAACAATAAATAAATAAAAAACTTAAAAACTGAACACGCGTG
TGTCCTCCTAACTCTGTATAATGGACAGGTAAAATGGATAAGAGTGTTATACCTGGATTT
TGCAGCTTTTGCCAAGGACCAAGAGCAACTAATATCTGCCCAGGGCCACAAATTCGATTA
CATAGAAGGGTTTGTGATAATAAACAGGACAGGCCTCCTGAACAGCTGGAGGTTGTCTTT
CACCGCAGAAGAGCCTTTAGAAGCAAGCCAATTCAAGTTTGATGGAAGGACTCTGTATTG
TCTGGAGCTAGCCAAGTATTTGAAGCAAGATAACAAAGACGTAATCAACCAGGTGAGAAA
ACAGAGTAGAAGCAATCGGTAGAATCTTCTTTGGTAGATGACATTCATTGGAACTGAAAA
TATATATATATTTGTCCAATCCAGGAAGTGAAAGAAACATTATCAGAGCTAAGCTACGTG
AATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAG
   
Sequence Name 58-K103677-0022-640-B04C-8409
GenBank Accession LN509681 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K103677-0022-640-B04C-8409
CATTTACAATTGAATATATTCACGTAGCTTAGCTCTGATAATGTTTCTTTCACTTCCTGG
ATTGGACAAATATATATATATTTTCAGTTCCAATGAATGTCATCTACCAAAGAAGATTCT
ACCGATTGCTTCTACTCTGTTTTCTCACCTGGTTGATTACGTCTTTGTTATCTTGCTTCA
AATACTTGGCTAGCTCCAGACAATACAGAGTCCTTCCATCAAACTTGAATTGGCTTGCTT
CTAAAGGCTCTTCTGCGGTGAAAGACAACCTCCAGCTGTTCAGGAGGCCTGTCCTGTTTA
TTATCACAAACCCTTCTATGTAATCGAATTTGTGGCCCTGGGCAGATATTAGTTGCTCTT
GGTCCTTGGCAAAAGCTGCAAAATCCAGGTATAACACTCTTATCCATTTTACCTGTCCAT
TATACAGAGTTAGGAGGACACACGCGTGTTCAGTTTTTAAGTTTTTTATTTATTTATTGT
TTACCATGGTTGGTGCTGGTTCCAATGCTATTCTTGCCCGCGTTATGATGCCAAACTGAC
CTAAACCACCAAGAACACCATTAAATAAGTCGCTGTTCTGCCTCTTTGTACAGTTTAGGA
TCT

Last Updated on 06.12.2018 16:11