Sequences from confirmation amplicon


Confirmation sequences for line 641C01 and hit At1g19660 are shown.
Line ID 641C01
Locus At1g19660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6801160
Primers used for PCR 18an - 8474, show primer details
   
Sequence Name 36-K107114-0022-641-C01C-8409
GenBank Accession LN502243 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K107114-0022-641-C01C-8409
ATTTACATTGAATCTCATCATACCAAATGAATGGAAGATGCATTTCTATGCCAGAAGTTA
CAAACCTTTGAAAGGTATAACTCTGCGAAATATGCCTCGTGAACTCTTGTGGTAACTCTA
ACAAGCCTAACCTGCAGAAAAAGTTGGTGTTTGGTAACGTTACTCACATAAGAATTTGTA
TTGCTAAAGGGAAAATAACAGGATAGAGAAAACAGAAAAACCGAAAGGCGTACCTCATAA
CCCATTTTGTCTACCATGTCCTTCACCACTTGATACATAGTGGGCCTTGCCTACAAGCAG
TAACCAAAAAAAATCCATAAACCCTCTCTTCACAACTCTTGACTGATTCAAACAGAAGCA
TAACGCAAAGAATAACAGAAGCCCTACTATTTGAACATTTGTCATTGCTGCCATAAGTAA
CACAC
   
Sequence Name 52-K107115-0022-641-C01C-18an
GenBank Accession LN507834 [GenBank]
Sequencing primer gene specific primer, 18an: 5'-AGTGTGTTACTTATGGCAGCAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K107115-0022-641-C01C-18an
CTTCTGTTGAATCAGTCAAGAGTTGTGAAGAGAGGGTTTATGGATTTTTTTTGGTTACTG
CTTGTAGGCAAGGCCCACTATGTATCAAGTGGTGAAGGACATGGTAGACAAAATGGGTTA
TGAGGTACGCCTTTCGGTTTTTCTGTTTTCTCTATCCTGTTATTTTCCCTTTAGCAATAC
AAATTCTTATGTGAGTAACGTTACCAAACACCAACTTTTTCTGCAGGTTAGGCTTGTTAG
AGTTACCACAAGAGTTCACGAGGCATATTTCGCAGAGTTATACCTTTCAAAGGTTTGTAA
CTTCTGGCATAGAAATGCATCTTCCATTCATTTGGTATGATGAGTATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11