Genotyping and primer details


Genotyping and primer details for 641C01 - At1g19660
Line ID 641C01
Locus At1g19660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6801160 of line 641C01 Chr1:6801160
   
Insertion allele amplicon 8474 - 18an
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18an: 5'-AGTGTGTTACTTATGGCAGCAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6801561
   
wt amplicon (over InsPos) 53vh - 18an
wt amplicon start Chr1:6800906
wt amplicon end Chr1:6801561
wt amplicon size 656 bp
Locus specific primer 18an: 5'-AGTGTGTTACTTATGGCAGCAATG-3'
2nd locus specific primer 53vh: 5'-GTTAAGATGATCTCATGGAGAGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11