Sequences from confirmation amplicon


Confirmation sequences for line 642A10 and hit At3g23870 are shown.
Line ID 642A10
Locus At3g23870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8621051
Primers used for PCR 24pk - 8474, show primer details
   
Sequence Name 33-K115038-0022-642-A10C-24pk
GenBank Accession LN501111 [GenBank]
Sequencing primer gene specific primer, 24pk: 5'-AAAGAGAAATTGCATATGTTTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115038-0022-642-A10C-24pk
CTATAGTCTTACACGCACCACATGAACAGAAAATAGAATCAGTCAAGCAAATATGGCAGC
TTGCTATTGAACCAGGTATATTTTCATAAGTACCGCAAAGTTTTGGTGTGCAAAATCGAT
TCTAGTGAAGATTGATATATATATGAAAGTTCTGTTTCATCAGGTTTCCTTGTGTATTCT
GCTGTGATTGTGATTGTGGTGGCTATACTGATCTTCTACTATGAACCGCGCTACGGGAAG
ACTCATATGATCGTATATGTCGGAATCTGCTCCTTAATGGGTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 23-K115037-0022-642-A10C-8409
GenBank Accession LN496672 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K115037-0022-642-A10C-8409
AGCAGATTCCGACATATACGATCATATGAGTCTTCCCGTAGCGCGGTTCATAGTAGAAGA
TCAGTATAGCCACCACAATCACAATCACAGCAGAATACACAAGGAAACCTGATGAAACAG
AACTTTCATATATATATCAATCTTCACTAGAATCGATTTTGCACACCAAAACTTTGCGGT
ACTTATGAAAATATACCTGGTTCAATAGCAAGCTGCCATATTTGCTTGACTGATTCTATT
TTCTGTTCATGTGGTGCGTGTAAGACTATAGTTGTAGACCCAACAACACAGAGAATGCAA
CCAAGTATCCCAAACATATGCATTTTTCTCTT

Last Updated on 06.12.2018 16:11