Sequences from confirmation amplicon


Confirmation sequences for line 643C10 and hit At1g61810 are shown.
Line ID 643C10
Locus At1g61810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22830879
Primers used for PCR ND12 - 8474, show primer details
   
Sequence Name 04-K032412-022-643-C10C-8409
GenBank Accession LN487802 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K032412-022-643-C10C-8409
TTACATTGAAAACTATTCGTCGATTGAGTTTCAGTATTTTAATTTATCAACTCTGGTTAG
TATATGAGATGCAATCGAATATTTTCTTTTTTTTACCATGCAATGCAATGTAGTATTAAT
AATACTGCTGATTTAACTAAAGAAGGACTTACTAGGTAAGATTCTACACCAAGAAATTGA
AAACCTATAACTGTTCACTCCAAGAAAAGACATTAATTGGATGTCCTCCTAAGCATAGAA
GATAATATAAAATAAATAACAACATCAAATAATATGAGAATATAATATGATAAAAGAAGT
TGACAAGAACAAAAATAGAAAGAAATAATTATCATAAGAAAATAACCAAAAATCGATTAT
ATTGGTCCACAGCTCTATCTGCATTGTTCTTGTCAAGTATTTTCCCTGCAGTTCCAATAT
ATATACTTGTCAATAGAATAAGTGAATTAAAAAAAAATAAAAATCTTAAACTAATCTTAA
ACTATTTCAAATAGAAAATAAACCAAATGGAAACCAAAATAAAATACATACCAGGATTTT
TATGCGTAAAGACATCCCAT
   
Sequence Name 04-K032412-022-643-C10C-ND12
GenBank Accession LN487803 [GenBank]
Sequencing primer gene specific primer, ND12: 5'-AAAAGTTTGAACAATTGGGATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K032412-022-643-C10C-ND12
TATTTTGGTTCCATTTGGTTTATTTTCTATTTGAAATAGTTTAAGATTAGTTTAAGATTT
TTATTTTTTTTTAATTCACTTATTCTATTGACAAGTATATATATTGGAACTGCAGGGAAA
ATACTTGACAAGAACAATGCAGATAGAGCTGTGGACCAATATAATCGATTTTTGGTTATT
TTCTTATGATAATTATTTCTTTCTATTTTTGTTCTTGTCAACTTCTTTTATCATATTATA
TTCTCATATTATTTGATGTTGTTATTTATTTTATATTATCTTCTATGCTTAGGAGGACAT
CCAATTAATGTCTTTTCTTGGAGTGAACAGTTATAGGTTTTCAATTTCTTGGTGTAGAAT
CTTACCTAGTAAGTCCTTCTTTAGTTAAATCAGCAGTATTATTAATACTACATTGCATTG
CATGGTAAAAAAAAGAAAATATTCGATTGCATCTCATATACTAACCAGAGTTGATAAATT
AAAATACTGAAACTCAATCGACGAATAGTTATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11