Sequences from confirmation amplicon


Confirmation sequences for line 644E11 and hit At1g48030 are shown.
Line ID 644E11
Locus At1g48030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17718906
Primers used for PCR 24kq - 8474, show primer details
   
Sequence Name 42-K113872-0022-644-E11C-24kq
GenBank Accession LN504537 [GenBank]
Sequencing primer gene specific primer, 24kq: 5'-AGAGCCTCTCCTCTTTACATTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113872-0022-644-E11C-24kq
AGTCTCTCTTTCCTCTAAACAACATGGCGATGGCGAGTTTAGCTAGGAGGAAGGCGTATT
TTCTCACCAGAAACTTATCAAACTCTCCCACTGACGCTCTCAGATTCTCCTTTTCCCTCT
CCCGTGGCTTCGCCTCATCAGGATCTGATGAAAACGACGTCGTCATCATCGGCGGCGGTC
CCGGTGGTTACGTAGCCGCGATCAAAGCCTCTCAGCTTGGTCTCAAAACCACTTGTATCG
AGAAACGCGGCGCTCTCGGTGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACCGATTATT
   
Sequence Name 16-K113871-0022-644-E11C-8409
GenBank Accession LN493510 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K113871-0022-644-E11C-8409
TCGGACTGAGCATTTCATTGATATTTCCCCGAGAGCGCCGCGTTTCTCGATACAAGTGGT
TTTGAGACCAAGCTGAGAGGCTTTGATCGCGGCTACGTAACCACCGGGACCGCCGCCGAT
GATGACGACGTCGTTTTCATCAGATCCTGATGAGGCGAAGCCACGGGAGAGGGAAAAGGA
GAATCTGAGAGCGTCAGTGGGAGAGTTTGATAAGTTTCTGGTGAGAAAATACGCCTTCCT
CCTAGCTAAACTCGCCATCGCCATTGTTGTTTTAGAGAGAAAGAGAGACTTGGCTCCTTG
TAGAGATGAACAGAGCCGGCAAATGTAAAGAGGGGAGGCTC

Last Updated on 06.12.2018 16:11