Sequences from confirmation amplicon


Confirmation sequences for line 645D01 and hit At3g04040 are shown.
Line ID 645D01
Locus At3g04040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1048516
Primers used for PCR CH98 - 8474, show primer details
   
Sequence Name 33-K107033-0022-645-D01C-8409
GenBank Accession LN501018 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K107033-0022-645-D01C-8409
GAGCCATTTACAATTGAATATATCCTGACCAGTTACATGAATATATGAAGTTTCAACAAC
ACAACAACCCAAAGAGAGATTCTGAATGTTTTTCAAGCAAGTTCCAAACTTTAAACTGTT
GTTTCCTCTATTCTGACTTAAAAAACATATCACACCACAAAAAGAATGTCGATTGTTTCA
GCATTAACCACACAAATCGAGCATCACATTCACCCCGACCTTTTCTTAGAACAGGTTGCA
TATATATTTTACCCTCTTAAGTCATTAAAAGAAGAAACAATCAATGATGGGACACCAATT
CACAAGAAGATACCAACACAAAGAAATCAATCAGTGAGAGATTAAAAGAAATACCATGGT
GAAACCCAATGAAAGCACTTGCAGTACCACCAACAGCTGATCCAATCTAATTAATCAATA
ACAAATCATAAATGACTAATAACCATTTGATCCAAGCAAAAAAACAGAAACTTGTTCTGA
GCAACTATTAGCCTTAGCAAGGTATAGTGTAAAAGAGTCGATTTTTAGCACCAAAATACA
AAAATTTCG
   
Sequence Name 41-K107034-0022-645-D01C-CH98
GenBank Accession LN504094 [GenBank]
Sequencing primer gene specific primer, CH98: 5'-GACGAAATTTTGTATTTTGGTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K107034-0022-645-D01C-CH98
CCTTGCTAGGCTATAGTTGCTCAAACAAGTTTCTGTTTTTTTGCTTGGATCAAATGGTTA
TTAGTCATTTATGATTTGTTATTGATTAATTAGATTGGATCAGCTGTTGGTGGTACTGCA
AGTGCTTTCATTGGGTTTCACCATGGTATTTCTTTTAATCTCTCACTGATTGATTTCTTT
GTGTTGGTATCTTCTTGTGAATTGGTGTCCCATCATTGATTGTTTCTTCTTTTAATGACT
TAAGAGGGTAAAATATATATGCAACCTGTTCTAAGAAAAGGTCGGGGTGAATGTGATGCT
CGATTTGTGTGGTTAATGCTGAAACAATCGACATTCTTTTTGTGGTGTGATATGTTTTTT
AAGTCAGAATAGAGGAAACAACAGTTTAAAGTTTGGAACTTGCTTGAAAAACATTCAGAA
TCTCTCTTTGGGTTGTTGTGTTGTTGAAACTTCATATATTCATGTAACTTGGTCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCG

Last Updated on 06.12.2018 16:11