Sequences from confirmation amplicon


Confirmation sequences for line 645G04 and hit At1g05780 are shown.
Line ID 645G04
Locus At1g05780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1727257
Primers used for PCR 42sj - 8474, show primer details
   
Sequence Name 37-K116518-0022-645-G04C-42sj
GenBank Accession LN502749 [GenBank]
Sequencing primer gene specific primer, 42sj: 5'-TGAATGTGAAGATCATCAAAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K116518-0022-645-G04C-42sj
AGTTTCAAAAGTTGTACAATAGAGAAAATTTCTAAAACTACCACCATAGCCAAGTAAAAG
GAACTATTAAACTGCTTTCATGAGAAAAGTTCTACATAAAAGAGTGTACGTAGAAGCCAA
AGATTTCAAGAATTTGTGTAGTTTATGATCTCCAGCGAAGAAGATGAAGTTGTTTTGCTT
CAAGGCATCATTGACTTCACTCATTAGGAGTGGAAACCCTAGTAAGAATATATATGATTT
TTATGAGTTTGTGAAGATCTCTCTAGCTATAATGATAAGGATCTTTCTGGCTTTTCATCT
TCTTCATCTTTGACCCTTCCTTCAAGTTGTCCTAGCGCATCTTCTTTCAATTTCTCAAAC
CTTCCTCGACAAATCATAACGACATGGGCCAAAAGGTATCGGAAACCCAATCTCTACTTC
AGTCTTTCCCTTTTGGAAAGAGAGAGACTGAAGAAGAGGTTGAGTTTTCGAAACCTTTTA
ACCAAAGTTGTTATGATTTGTCAAGAAAGATTTAGGGTATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 11-K116517-0022-645-G04C-8409
GenBank Accession LN491283 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116517-0022-645-G04C-8409
TTGAATATACCCTAAATCTTTCTTGACAAATCATAACAACTTTGGTTAAAAGGTTTCGAA
AACTCAACCTCTTCTTCAGTCTCTCTCTTTCCAAAAGGGAAAGACTGAAGTAGAGATTGG
GTTTCCGATACCTTTTGGCCCATGTCGTTATGATTTGTCGAGGAAGGTTTGAGAAATTGA
AAGAAGATGCGCTAGGACAACTTGAAGGAAGGGTCAAAGATGAAGAAGATGAAAAGCCAG
AAAGATCCTTATCATTATAGCTAGAGAGATCTTCACAAACTCATAAAAATCATATATATT
CTTACTAGGGTTTCCACTCCTAATGAGTGAAGTCAATGATGCCTTGAAGCAAAACAACTT
CATCTTCTTCGCTGGAGATCATAAACTACACAAATTCTTGAAATCTTTGGCTTCTACGTA
CACTCTTTTATGTAGAACTTTTCTCATGAAAGCAGTTTAATAGTTCCTTTTACTTGGCTA
TGGTGGTAGTTTTAGAAATTTTCTCTATTGTACAACTTTTTGAAACTGTACTCTCTTAGA
ATTGTCATATCAATATAGCTTTTTGATGATCTCCACATTCA

Last Updated on 06.12.2018 16:11