Sequences from confirmation amplicon


Confirmation sequences for line 646C02 and hit At5g39860 are shown.
Line ID 646C02
Locus At5g39860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15958321
Primers used for PCR KY02 - 8474, show primer details
   
Sequence Name 27-K026963-022-646-C02C-8409
GenBank Accession LN498230 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K026963-022-646-C02C-8409
TAGATTTCCCGGACATGAAGCCATTTACAAAAGAACAAAAAAGATTACATGAGTAGGCTT
CTAATAACGGCGGCTTCAGGGCTATCTTCATCGACAGATTCGAGAAGCTGAGACAAACGC
TCGCTCAGATTGTCAACTTCTCTGTTCAAATTTCGTATGTAATTGCATGTCTCTTGCAAT
ACTTTCGAGGCTGATGCCTTCAACAACAAAAACGAAATAAAATCATAATCATTATAATAT
ATGTTTTTTGAACCAATGCATGCGCTTTTATGCGTTTGCAGATGCATGCAAGCTGATGAT
TTTGATGTAATCTCTTATGTAAATATGGAAGAAAAAGGTTGTGAATATTGGTATATATGT
TCCTTAATGTCCGGAGTCAAAAGTTCCGACTTCTTTTTTCTTTCTGGTTTGATTTTAGTG
TCCACTCTTCTTTTTACAAAACTTTTGTTCTTTCCTTTTTTGGATAGGTTTACACGATGG
ATAATGTTAAATGGTCGTTATAAATGAATATATGCATATATTATG
   
Sequence Name 31-K026963-022-646-C02C-KY02
GenBank Accession LN500011 [GenBank]
Sequencing primer gene specific primer, KY02: 5'-CGTTCTGATAAGGTATCACGCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K026963-022-646-C02C-KY02
ATATATGCATATATTCATTTATAACGACCATTTAACATTATCCATCGTGTAAACCTATCC
AAAAAAGGAAAGAACAAAAGTTTTGTAAAAAGAAGAGTGGACACTAAAATCAAACAAGAA
AGAAAAAAGAAGTCGGAACTTTTGACTCCGGACATTAAGGAACATATATACCAATATTCA
CAACCTTTTTCTTCCATATTTACATAAGAGATTACATCAAAATCATCAGCTTGCATGCAT
CTGCAAACGCATAAAAGCGCATGCATTGGTTCAAAAAACATATATTATAATGATTATGAT
TTTATTTCGTTTTTGTTGTTGAAGGCATCAGCCTCGAAAGTATTGCAAGAGACATGCAAT
TACATACGAAATTTGAACAGAGAAGTTGACAATCTGAGCGAGCGTTTGTCTCAGCTTCTC
GAATCTGTCGATGAAGATAGCCCTGAAGCCGCCGTTATTAGAAGCCTACTCATGTAATCT
TTTTTGTTCTTTT

Last Updated on 06.12.2018 16:11