Sequences from confirmation amplicon


Confirmation sequences for line 646F05 and hit At4g34220 are shown.
Line ID 646F05
Locus At4g34220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16383546
Primers used for PCR CS65 - 8474, show primer details
   
Sequence Name 03-K104856-0022-646-F05C-CS65
GenBank Accession LN487480 [GenBank]
Sequencing primer gene specific primer, CS65: 5'-CAAAGGGTTTATTGGAGCTGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K104856-0022-646-F05C-CS65
AATTTGGAGGGTTTGAAGAGTGGAAGGAATTGAACAAAGGATCTTCAATGGCTTCCCACA
AAGCTCTTGGTTACCGGAAAAAGACTCAGCCTTTTGGTTAAGTAAAGAAAGGGAACTTGG
GATTGGGCCTGTGAGGTTGTTAAAGGAGAGATCGACTGTGGCATTTGCTGGAAACTTCTC
TGCAAAATTTGGAGAAATCTCGCCGAGGACTTTGTTGTGTGATAAGTTCAAGTAATGCAG
GCTTTTTCCTCCCAAATCCTTGGGAAGAGAGCCGTTGAGAAGATTTGAAGAAAGATCAAG
AATCTGTGCTGCTTCGAAACCACTTGGAATATCACCTGAAAAGGTGTTTTTTGACAAGGA
AACGACCGTTAGATTCTTCAGAAGTGAGATGTTGACACTTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTCC
   
Sequence Name 19-K104857-0022-646-F05C-8409
GenBank Accession LN494752 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104857-0022-646-F05C-8409
CCATTACATTGAAGTGTCACATCTCACTTCTGAAGAATCTAACGGTCGTTTCCTTGTCAA
AAAACACCTTTTCAGGTGATATTCCAAGTGGTTTCGAAGCAGCACAGATTCTTGATCTTT
CTTCAAATCTTCTCAACGGCTCTCTTCCCAAGGATTTGGGAGGAAAAAGCCTGCATTACT
TGAACTTATCACACAACAAAGTCCTCGGCGAGATTTCTCCAAATTTTGCAGAGAAGTTTC
CAGCAAATGCCACAGTCGATCTCTCCTTTAACAACCTCACAGGCCCAATCCCAAGTTCCC
TTTCTTTACTTAACCAAAAGGCTGAGTCTTTTTCCGGTAACCAAGAGCTTTGTGGGAAGC
CATTGAAGATCCTTTGTTCAATTCCTTCCACTCTTTCAAACCCTCCAAATATCTCTGAAA
CTACTTCACCAGCAATAGCAGTTAAACCAAGAAGTACAGCTCCA

Last Updated on 06.12.2018 16:11