Sequences from confirmation amplicon


Confirmation sequences for line 646F06 and hit At3g05910 are shown.
Line ID 646F06
Locus At3g05910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1765931
Primers used for PCR NB59 - 8474, show primer details
   
Sequence Name 14-K034548-022-646-F06C-NB59
GenBank Accession LN492412 [GenBank]
Sequencing primer gene specific primer, NB59: 5'-TGTTTGATGTTTTTGTATCAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K034548-022-646-F06C-NB59
GACCTGTGTATACAGGAAGACACTCGACGTGGTTCCTCTAATTATATGGAGAAACAGCTT
CAGTTTACAGGAATACTGAGTGACAAAGCTCAAGAAAATCCTGGTTTGTCTCAATCTTTG
TTTAAGTTTTGTTCATGTTAGTGTAATGTTAATATTGAAAATCATATATTCTCATTGTCA
TGTGCTTTACTTCTGCAGACTTTTTCAACTGGAATAGAGTTAAGCTTCGTTACTGCGATG
GCGCTTCTTTCAGTGGAGATGGCCAGAATCAGGTTGAAATCAATCATTTTTTGAGTTACT
AGAGTTCATAAGAGACCCATTGTTGTGATTTGGAACAACTTGCTAGTTTCTAGGCCTTTG
GAACTCTGTAGTCTATAATCGTCAAGGCTGTTATTAGCCACAGAGAACGCTTGATGTGAT
CTTAGTTCTGTAGAATTGAGCAGCCATTGGTTTTGTGTAACATCTGTGAACTAATGCAAA
TAAAATTCTTTACCTTAACTTTTTTATGATTGATGAATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 13-K034548-022-646-F06C-8409
GenBank Accession LN491938 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K034548-022-646-F06C-8409
CGGACATGAGCCATTTACAATTGAATATTCATCAATCATAAAAAAGTTAAGGTAAAGAAT
TTTATTTGCATTAGTTCACAGATGTTACACAAAACCAATGGCTGCTCAATTCTACAGAAC
TAAGATCACATCAAGCGTTCTCTGTGGCTAATAACAGCCTTGACGATTATAGACTACAGA
GTTCCAAAGGCCTAGAAACTAGCAAGTTGTTCCAAATCACAACAATGGGTCTCTTATGAA
CTCTAGTAACTCAAAAAATGATTGATTTCAACCTGATTCTGGCCATCTCCACTGAAAGAA
GCGCCATCGCAGTAACGAAGCTTAACTCTATTCCAGTTGAAAAAGTCTGCAGAAGTAAAG
CACATGACAATGAGAATATATGATTTTCAATATTAACATTACACTAACATGAACAAAACT
TAAACAAAGATTGAGACAAACCAGGATTTTCTTGAGCTTTGTCACTCAGTATTCCTGTAA
ACTGAAGCTGTTTCTCCATATAATTAGAGGAACCACGTCGAGTAGTCTTCCTGTATACAC
AGGTCCTGATGTTGTTGCACCATCCTCCTCCCTGAT

Last Updated on 06.12.2018 16:11