Sequences from confirmation amplicon


Confirmation sequences for line 647B01 and hit At1g26480 are shown.
Line ID 647B01
Locus At1g26480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9157364
Primers used for PCR 37ms - 8474, show primer details
   
Sequence Name 28-K114240-0022-647-B01C-37ms
GenBank Accession LN498948 [GenBank]
Sequencing primer gene specific primer, 37ms: 5'-TTGAAACCTTTCAGGAGAGTTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114240-0022-647-B01C-37ms
AGAGCAGGCCAAGACGAATTGGATGGGTCGAAGGAAGCTCAGTGCTTGCAGCTTGTGTTG
CAGCCTAATCAAAATACAGATGCATCAGACCAAACTTCTCATAATTCTCTAGTATTTGAC
TAAAGAAACAAATTTAATACCTCATAGCCTTTGAGAGACTGTTCAGCAGCTTCCTTCCTC
TCTTGCTCGGTCTTAAACTCAGCCAAGTAACGGTAATAATCTCCTTTCCTACAAATTCCA
GATCAAATTGAGTTTCATTTGCAGATGATGATTCAAAAAGAAGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 04-K114239-0022-647-B01C-8409
GenBank Accession LN488008 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114239-0022-647-B01C-8409
CATTTCATTGAATATATCTTCTTTTTGAATCATCATCTGCAAATGAAACTCAATTTGATC
TGGAATTTGTAGGAAAGGAGATTATTACCGTTACTTGGCTGAGTTTAAGACCGAGCAAGA
GAGGAAGGAAGCTGCTGAACAGTCTCTCAAAGGCTATGAGGTATTAAATTTGTTTCTTTA
GTCAAATACTAGAGAATTATGAGAAGTTTGGTCTGATGCATCTGTATTTTGATTAGGCTG
CAACACAAGCTGCAAGCACTGAGCTTCCTTCGACCCATCCAATTCGTCTTGGCCTTGCTC
TTAACTTCTCTGTTTTCTACTATGAGATCATGAACTCTCCTGAAAGGTTTCA

Last Updated on 06.12.2018 16:11