SimpleSearch - Line and FST details
Line specific information
| Line ID | 647B01 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N462029) |
| Segregation Analysis | 34:7:7 |
| Confirmed for Hit | At1g26480 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 7282, 9946, 10897, 72368, 72382, 72397, 72398, 72399, 72400, 72401, 72402, 72403 |
| Note |
Gene hit At5g63740
| Sequence (A. th genome BLAST matches underlined) | >02-K023276-022-647-B01-8409 CATTTACAATTGAATAAATCACTGGTAGGAAAACAAGATGGATTCGATCATATTGACTGT AAATAATGAATATAAATGGCAAGAACGATGATGTTTCATATATATAGAGTTTGGAAAGAC TATTACACTGATCAGCTCGACATCCTCGATAATTTAAAAATAAAATAAAAAACGGGAATA AATGCATTTCATAAAAGAAGAACCAAATTTTCCTTTTGAGTAAATTCAAATTAGGTCAAA GATAATGGCCTATGGGATCCTCCCTATAATGAGAGGAGAACACACCGAATCGCAGATACC CCAGTTCATCCTGTTGCTCCGATATAAATTTCCCGAAGGCGTTCATATACCATTTGATCC TGGGCAAAATACGATCCCCAACCAGCCGCCGGACCGACGCGGGCAAACAAAACCCAACCA AGCGAGCTCCAGGGAGTCGAAAACCGGCGGAGCCAGCCTCTCGTGAGGGAAAGACCGCAC AACGGCAAACTGAACCGGGGCCGGGGCCCCCTCCCGAAGCCGAAGATACCCATTTCATCT TCAGATTGGACN |
| GenBank Accession | FR813972 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:25510562 - go to primer design |
| BLAST e Value | 2e-45 |
| Hit Clone Code (BAC ID) | MBK5 |
| Hit Gene Code | At5g63740 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | RING/U-box superfamily protein |
| Insertion Classification | 3' region |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | FR813971 [GenBank] BX893457 [GenBank] |
Gene hit At1g26480
| Sequence (A. th genome BLAST matches underlined) | >02-K022672-022-647-B01-8409 TTCACGGACTTGATTCCATTTACCATTGAATATATCCTGCTTTATGTTAATCATGTGTTA AAGGAACTGACTTTGATCTCGCCTCTTGATGGAATGAGATACATACCGCCACTGGACGGA TCTCTGCTACACAATCACTACAAAGCGATCCTCGCTCACAATCGTTCGCATGCCATAAAG CATTAGCCTTGTTTCCCTTTCCTATACTACATATTTATTCTCCATTTGGGCCATTCTGCC TTCTTCTGCTGAGACGGCCTCGCTCCCTCCCGGCGCTCAGAGCCCTTCGCCCCACCTCAC TCTTCTTACCCTCGCTATAAAGCTCTCTCATGTCAACCTCCCTATCNTGAGCTACACCGA AAGGTTTCCATCTACAACGTCATCTTTTAAATATACCAATAGATTGTCGGCTTTTTTGTA ATGTGGTTTCTATTAAACTATTGCTGAACTTGCCATTT |
| GenBank Accession | BX659610 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:9157376 - go to primer design |
| BLAST e Value | 1e-15 |
| Hit Clone Code (BAC ID) | T1K7 |
| Hit Gene Code | At1g26480 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | general regulatory factor 12 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At3g12580
| Sequence (A. th genome BLAST matches underlined) | >02-K023295-022-647-B01-8409 TTGAATATATCCTGGGAAAAAAAGACCCATCTCGAAGGAGCTATGGGATCCTCCCTATAG TGAGTCGGATTACTCCCGCTGATATGCCTAGATGGATATACAGGGAGTATGAATATATGG GGATTGCGATGGTNNATTTTTTAAATTTATAAAAAATCGGGATTACTCCCTCCTTACCCA GGGGGGGGGGGGGGGCTTCCCCCCCCCCCCAAACCCCCGCCGACCCCCGCCCTTCACTCG CCACACCCCCGGAACAACCAAACACTCCACCGCGGTGCCCGCGCCTCGCCCAAACCCCCA GATACCCATTTCATCTTCAGATTGGTCTGAGATTATGCCAAAATATACCCTCATATACAT AAATACTGACAGTTTGAGCTACCAATTCAGTGTAGCCCA |
| GenBank Accession | FR813973 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr3:3993967 - go to primer design |
| BLAST e Value | 0.052 |
| Hit Clone Code (BAC ID) | T2E22 |
| Hit Gene Code | At3g12580 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | heat shock protein 70 |
| Insertion Classification | Promoter |
| Confirmation Status | failed |
| Other FSTs Supporting this Hit | FR813973 [GenBank] |
|
Last Updated on 10.06.2021 13:37 |
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